HLA-DRB1等位基因与四川汉族人寻常型天疱疮的相关性研究

目的 探讨HLA-DRB1等位基因与四川汉族人寻常型天疱疮的相关性.方法 采用聚合酶连反应-序列特异性引物(PCR-SSP)对19例四川汉族寻常型天疱疮患者和25例健康对照组进行低分辨和高分辨HLA-DRB1等位基因分型,计算各等位基因频率.采用x2检验比较两组等位基因频率.结果 在寻常型天疱疮患者和健康对照者中共检出9种低分辨DRB1等位基因和19种高分辨DRB1等位基因.与健康对照组相比,寻常型天疱疮患者DRB1*14等位基因频率(39.47％,15/38)及DRB 1*1405等位基因频率(15.79％,6/38)均显著高于健康对照组8.00％(4/50),2.00％(1/50)],差异均有统计学意义(x2=17.43、4.25,均P＜0.05).结论 DRB1*14可能是四川汉族寻常型天疱疮患者的常见易感基因,其中DRB1*1405与寻常型天疱疮最具有相关性.

Association analysis between HLA-DRB1 alleles and pemphigus vulgaris in a Han population in Sichuan

Objective To investigate the association between HLA-DRB1 alleles and pemphigus vulgaris (PV) in a Han population in Sichuan.Methods Polymerase chain reaction with sequencespecific primer (PCR-SSP) method was used for low-resolution and high-resolution typing of HLA-DRB1 alleles in 19 patients of Han nationality with PV and 25 healthy controls in Sichuan.Allele frequencies were calculated,and differences in the allele frequency between the above two groups were compared by using chisquare test.Results Totally,9 kinds of DRB 1 low-resolution alleles and 19 kinds of DRB 1 high-resolution alleles were identified in the PV patients and healthy controls.Frequencies of the DRB1* 14 allele (39.47％15/38] vs.8.00％4/50],x2 =17.43,P ＜ 0.05) and DRB1*1405 allele (15.79％6/38] vs.2.00％1/50],x2 =4.25,P ＜ 0.05) were significantly higher in the PV patients than in the healthy controls.Conclusion The HLA-DRB1*14 allele may be a common susceptibility gene for PV in the Han population in Sichuan,and the HLA-DRB1* 1405 allele may be most closely associated with PV.