酶联免疫吸附试验在结核性脑膜炎诊断中的初步应用

结核性脑膜炎的诊断目前主要依据临床表现、CSF的异常改变、OT试验及X线和细菌学检查。由于疾病早期临床表现和CSF改变往往不典型;OT试验阳性有助于诊断,阴性不能除外结脑;X线仅用于有无合并脑外结核的检查。尽管从CSF中查到结核酶即可确定诊断,但阳性率太低。国内报导涂片阳性约15～30％,培养约30～40

Prelimnary Application of An Enzyme-linked Immunosordent Assay (ELISA) in Diagnosis of Tuberculosis Menigitis

Cerebrospinal fluid (CSF) and serum from patients with tuberculosis meningitis (TBM) and nontuberculosis meningitis were assayed for IgG antibody activity to purified protein derivative (PPD) by ELISA.The results showed that the patients with TBM clearly had higher levels of antibody to PPD antigen than did approrjate control groups (P<0.001).The sensitivity for the detection of antibody activity in CSF and serum was 90.9% and 81.8%,specificity 95.9% and 96.4% respectively by using this method. There was not a statistically significant difference of ELISA positive rate between the antibody in CSF and serum (P>0.05).Because of its sensitity, specificity,and rapidity value,this method can be used for early diagnosis of tuberculosis meningitis.