热休克蛋白27与NF-κBp65在喉鳞状细胞癌中的基因表达及临床意义

目的研究热休克蛋白27（heat shock protein27,HSP27）及核因子NF-κBp65（NF-κBp65）在喉癌及癌旁组织中基因表达水平,并探讨两者与喉癌临床病理因素的相关性。方法采用RT-PCR方法分别检测HSP27 mRNA和NF-κBp65 mRNA在喉癌组织及癌旁组织中的表达水平,并统计两者与喉癌不同临床病理因素的关系。结果 HSP27 mRNA和NF-κBp65 mRNA分别在喉癌组织中的表达高于癌旁组织,两基因与喉癌不同病理分型有相关性,与临床分期及有无淋巴结转移无关,且NF-κBp65 mRNA和HSP27 mRNA在喉癌中呈负相关性（r=-0.4367,P〈0.05）。结论 HSP27 mRNA和NF-κBp65mRNA表达水平随喉癌恶性程度的增高HSP27 mRNA表达量逐渐下降,而NF-κBp65 mRNA表达量逐渐升高,推测HSP27在人喉癌中可能负性调节NF-κB信号传导通路,HSP27有望成为喉鳞癌基因治疗新的作用靶点。

HSP27 and NF-kappaBp65 in the laryngeal squamous cell carcinoma of the gene expression

OBJECTIVE To study the gene expression of heat shock protein 27（HSP27） and nuclear factor NF-kappaB in human laryngeal carcinoma and adjacent tissues,and to explore their relationship with clinicopathological factors.METHODS RT-PCR were used to detect HSP27 and NF-kappa B mRNA in human laryngeal carcinoma and adjacent tissues.The relationship of expression level and the clinicopathological factors was studied.RESULTS The mRNA level of HSP27 and NF-kappa B in human laryngeal carcinoma was higher than that in the adjacent tissues.Both gene expressions in human laryngeal cancer were related with histological grade,but had no relation with the clinical stage and lymph node metastasis.NF-kappa B mRNA expression had a negative relation with HSP27 mRNA expression.CONCLUSION HSP27 may be a negative regulator of NF-kappa B in signal transduction pathway in human laryngeal carcinoma.HSP27 may be as a new target for gene therapy in human laryngeal squamous cell carcinoma.