血管生成素2和miR-126与糖尿病大血管病变的相关性

目的 探讨血管生成素2 （Ang-2）和miR-126与T2DM患者大血管病变的关系. 方法 检测241例T2DM患者和104名健康对照（NC）者的临床指标、不同时相的胰岛素及C-P水平,测定单核细胞趋化蛋白-1（MCP 1）、血管内皮舒张因子（一氧化氮,NO）、血清miR-126和Ang-2表达水平,分析Ang-2与血管内皮细胞功能、miR-126表达水平的关系. 结果 T2DM合并大血管病变（T2 DM＋CVD）、T2DM未合并大血管病变（T2DM）组Ang-2水平与NC组比较差异有统计学意义（P＜0.05）.与T2DM、NC组比较,T2DM＋ CVD组Ang-2水平升高,miR-126表达水平降低（P＜0.05）.Ang-2与MCP-1呈正相关（r=0.456,P＜0.01）,与miR-126、NO呈负相关（r=-0.517,P＜0.01；r=-0.327,P＜0.05）. 结论 Ang-2和miR-126与糖尿病大血管病变密切相关.miR-126负性调节Ang-2表达.

The relationship of angiopoietin-2 and miR-126 with diabetic macroangiopathy in type 2 diabetes

Objective To investigate the relationship of angiopoietin-2 （Ang-2） and miR-126 with macroangiopathy in patients with T2DM.Methods Clinical indicators,the diffrent same phase of insulin and C-P levels,and monocyte chemoattractant protein-1 （MCP-1）,vascular relaxing factor （nitric oxide,NO）,miR-126 and serum Ang-2 expression levels were determined in 241 T2DM patients and 104 normal control（NC） people,the correlation between Ang-2,vascular endothelial cell function,miR-126 expression levels was analyzed.Results Ang-2 level in diabetic macroangiopathy with large vessel disease （T2DM＋CVD） group and without large vessel disease （T2DM） group was significantly higher than in NC group（P＜0.05）.Ang-2 level in T2DM＋CVD group was higer than in T2DM group,but expression of miR-126 was lower in T2DM＋ CVD group than in T2DM and NC group （P ＜ 0.05）.Expression of Ang-2 was positively related with MCP-1 （r=0.456,P＜0.01）,and negatively related with miR-126 and NO （r=-0.517,P＜0.01 ;r=-0.327,P＜0.05）.Conclusion Ang-2 is closely related to diabetic macroangiopathy.miR-126 is negatively regulate Ang-2 expression.