| 反向Northern筛查冠心病血瘀证差异片段 |
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| 引用本文： | 杨保林,王阶,姜燕.反向Northern筛查冠心病血瘀证差异片段[J].中国中医基础医学杂志,2004,10(9):29-30,34. |
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| 作者姓名： | 杨保林 王阶 姜燕 |
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| 作者单位： | 1. 中国中医研究院,西苑医院,北京,100091
2. 天津天士力集团,天津,300402 |
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| 基金项目： | 广州中医药大学校科研和教改项目，广东省中医药管理局科研项目 |
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| 摘 要： | 目的:降低mRNA差异显示假阳性.方法:采用反向Northern杂交方法进行筛查差异片段的真实性.结果:差异片段再扩增后经检测符合杂交条件的有96条,反向杂交后,有10条DNA片段属于真实的差异片段.结论:反向Northern杂交是降低mRNA差异显示假阳性的有效方法.
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| 关 键 词： | 反向Northern 冠心病血瘀证 差异片段 |
| 文章编号： | 1006-3250(2004)09-0029-02 |
Screening the Authenticity of Differentia Fragment of Blood Stasis Syndrome in Coronary heart Disease with Reverse Northern |
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| YANG Bao lin ,WANG Jie ,JIANG Yan.Screening the Authenticity of Differentia Fragment of Blood Stasis Syndrome in Coronary heart Disease with Reverse Northern[J].Chinese Journal of Basic Medicine In Traditional Chinese Medicine,2004,10(9):29-30,34. |
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| Authors: | YANG Bao lin WANG Jie JIANG Yan |
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| Institution: | YANG Bao lin 1,WANG Jie 1,JIANG Yan 2 |
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| Abstract: | Objective:To decrease the false postive rate of differential display PCR.Mehtods:Screeing the differential display PCR result by reverse northern blotting met hod.Result:By DDRT PCR we got 96 bands.The reverse northern blot results showed ten of them were real differential display bands.Conclusion:Reverse northern blotting is a effective mathod to decrease false postive rate of DD PCR. |
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| Keywords: | reverse northern blotting Blood Stasis Syndrome in Coronary Heart Disease differential display PCR |
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