柴胡提取物对大鼠海马原代培养神经元5-HT3受体介导离子通道的影响

目的: 探讨柴胡提取物（Radix Bupleuri Extract，RBE）含药血清对抑郁情绪模型大鼠原代培养的海马神经元5-羟色胺3受体（5-HT₃R）通道电流的作用。方法：复制抑郁情绪大鼠模型，以柴胡提取物进行药物干预，采用旷场实验和糖水偏好实验评价模型, 制备各组大鼠血清并灭活除菌，对原代培养的大鼠海马神经元干预24 h后，采用全细胞膜片钳技术检测各组神经元5-HT₃R通道电流。结果:与正常组相比，模型组大鼠旷场实验总分显著性降低（P<0.01），糖水偏好系数显著降低（P<0.01），模型组大鼠血清孵育的海马神经元电流密度值明显增加（P<0.01）；与模型组相比，柴胡提取物组和氟西汀组旷场实验总得分明显提高（P<0.05，P<0.01），糖水偏好系数均明显增加（P<0.05，P<0.05），柴胡提取物和氟西汀含药血清孵育的海马神经元电流密度值均明显减少（P<0.01，P<0.01）。结论：柴胡提取物能改善抑郁情绪模型大鼠异常的5-HT3R通道电流，这可能是其发挥抗抑郁情绪的中枢机制之一。

Effect of Radix Bupleuri Extract on 5-HT3R Pathway in Primarily Cultured Rat Hippocampal Neurons

This study was aimed to investigate the effect of Radix Bupleuri extract (RBE) on 5-HT₃R channel currents of primarily cultured hippocampal neurons in depression emotion rats. Depression emotion model rats were duplicated. RBE was used for drug intervention. And then, the rats were evaluated by the open-field test (OFT) and the sucrose preference test. Serum of rats in each group was collected and then added into the primary cultured hippocampal neurons for 24 h. The 5-HT₃R channel currents were recorded by the whole-cell patch clamp. The results showed that compared with the normal group, the total score of OFT in the model group was significantly decreased (P < 0.01); the sucrose preference ratio decreased obviously (P < 0.01); and the current density value of primary cultured hippocampal neurons in serum of the model group was significantly higher (P < 0.01). Compared with the model group, the total scores of OFT in the RBE group and fluoxetine group increased significantly (P < 0.05, P < 0.01); the sucrose preference ratio also increased obviously (P < 0.05, P < 0.05); and current density value of the primary cultured hippocampal neurons in serum of the RBE group and fluoxetine group decreased significantly (P < 0.01, P < 0.01). It was concluded that RBE can effectively correct the abnormal 5-HT₃R channel currents of rats with depression emotion, which may be one of the central mechanisms in the treatment of depression emotion.