| 五倍子多糖相对分子质量和单糖组成的测定 |
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| 引用本文： | 龚力民,刘伟,卓海燕,张红刚,李森,何琴,林丽美,李顺祥. 五倍子多糖相对分子质量和单糖组成的测定[J]. 中国实验方剂学杂志, 2015, 21(24): 34-36 |
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| 作者姓名： | 龚力民 刘伟 卓海燕 张红刚 李森 何琴 林丽美 李顺祥 |
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| 作者单位： | 湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208,湖南中医药大学, 长沙 410208 |
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| 基金项目： | 湖南省"十二五"药学重点学科支持实验室开放研究项目(科函[2015]9号-2);湖南省大学生研究性学习和创新性实验计划项目(2013173);长沙市科技局课题(K1301005-31);国家中医药管理局"药用物学"重点学科;湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(ZYFT201310) |
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| 摘 要： | 目的:研究五倍子多糖精细组分的相对分子质量、单糖组成。方法:五倍子多糖经提取纯化后得到GCP-1,GCP-2和GCP-3 3种精细组分。用高效凝胶渗透色谱(HPGPC)分析其相对分子质量;采用三氟乙酸(TFA)水解和1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化的高效液相色谱(HPLC)分析其单糖组成。结果:HPGPC试验结果显示,GCP-1,GCP-2,GCP-3的相对分子质量分别为182,2.1×10~4,6×10~4;单糖组成试验表明,GCP-2和GCP-3的单糖组成类型基本一致,主要为半乳糖、半乳糖醛酸、葡萄糖醛酸等。结论:用HPGPC和PMP柱前衍生法测得五倍子多糖精细组分的相对分子质量和单糖组成,方法简便可行,可用于五倍子多糖的研究。
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| 关 键 词： | 五倍子多糖 相对分子质量 单糖组成 高效凝胶渗透色谱法 |
| 收稿时间： | 2015-06-15 |
Molecular Weight and Monosaccharide Components in Polysaccharides from Galla Chinensis |
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| GONG Li-min,LIU Wei,ZHUO Hai-yan,ZHANG Hong-gang,LI Sen,HE Qin,LIN Li-mei and LI Shun-xiang. Molecular Weight and Monosaccharide Components in Polysaccharides from Galla Chinensis[J]. China Journal of Experimental Traditional Medical Formulae, 2015, 21(24): 34-36 |
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| Authors: | GONG Li-min LIU Wei ZHUO Hai-yan ZHANG Hong-gang LI Sen HE Qin LIN Li-mei LI Shun-xiang |
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| Affiliation: | School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China,School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China and School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China |
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| Abstract: | Objective: To determine the relative molecular weight and monosaccharide components in polysaccharides from Galla Chinensis(GCP). Method: Three fine compositions, GCP-1, GCP-2 and GCP-3 were obtained after extracting and purification of GCP. High performance gel permeation chromatography(HPGPC) was used to analyze the average molecular weight;high performance liquid chromatography(HPLC) of trifluoroacetic acid(TFA) hydrolysis and 1-phenyl-3-methyl-5-pyrazolone(PMP) pre-column derivatization was used to analyze its monosaccharide components. Result: According to HPGPC, the molecule weights were 182, 2.1×104 and 6×104, respectively for GCP-1, GCP-2 and GCP-3.The test of monosaccharide components showed that monosaccharide components of GCP-2 and GCP-3 were basically the same, mainly as galactose, galacturonic acid and glucuronic acid, etc. Conclusion: The two methods of HPGPC and PMP pre-column derivatization to determine the fine compositions of GCP are simple and convenient and could be applied to analysis of the molecular weight and monosaccharide composition in GCP. |
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| Keywords: | polysaccharides from Galla Chinensis relative molecular weight monosaccharide components HPGPC |
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