基于连作障碍条件下穿山龙无机元素吸收规律及总皂苷含量分析

目的:探讨不同连作条件对于穿山龙的无机元素吸收及总皂苷含量的影响。方法:采用电感耦合等离子体质谱(ICP-MS)对不同连作条件下的穿山龙药材与根际土壤中17种无机元素的含量进行测定,射频功率1.3 k W,等离子体流量15 L·min~(-1),载气流量1.16 L·min~(-1),采样深度8.6 mm,雾室温度2℃,分析时间3 s,测量数3次,排风量5.0~7.5 m3·min~(-1)(4.7~8.1 m·s~(-1));循环水的要求为流速5 L·min~(-1),温度15~20℃,压力230~350 k Pa。利用紫外分光光度法测定穿山龙中总皂苷含量,检测波长457 nm,通过SPSS 17.0统计软件对实验数据进行相关性分析。结果:穿山龙在正茬2~4年时,药材与土壤的无机元素呈显著相关;从连作一茬1年开始无明显相关性,药材中总皂苷含量亦呈下降趋势。结论:穿山龙随着连作年限的增加,其总皂苷的含量随之下降,说明连作障碍对药材中总皂苷的含量积累造成了影响,但与无机元素的变化无明显相关,说明无机元素只是造成连作现象的因素之一。

Absorption Law of Inorganic Elements and Contents of Total Saponins in Dioscoreae Nipponicae Rhizoma Based on Continuous Cropping Obstacles Conditions

Objective: To investigate effect of different continuous cropping conditions on inorganic elements absorption and content change of total saponins in Dioscoreae Nipponicae Rhizoma. Method: Seventeen kinds of inorganic elements in Dioscoreae Nipponicae Rhizoma and rhizosphere soil by inductively coupled plasma mass spectrometry(ICP-MS).Determination of total saponins in Dioscoreae Nipponicae Rhizoma was ultraviolet spectrophotometry.Data were analyzed by SPSS 17.0 software. Result: Contents of inorganic elements in herbs of Dioscoreae Nipponicae Rhizoma which was in rotation 2,3,4 years significantly correlated with soil.Continuous cropping stubble 1 years began to no significant correlation,and the content of total saponins showed downward trends. Conclusion: The content of total saponins in Dioscoreae Nipponicae Rhizoma is declining with increasing of continuous cropping time.These results show that continuous cropping affects the content of total saponins,but its content change has no obvious correlation with inorganic elements,which indicates that change of inorganic elements is just a factor for continuous cropping.