Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody |
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| Authors: | Zhen Liu Aimin Zhi Lina Zhao Yanyan Yang Xiaofei Hu Ruiguang Deng |
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| Affiliation: | 1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Science, Zhengzhou, Henan, PR China;2. College of Biotechnology, Henan University of Technology, Zhengzhou, Henan, PR China;3. College of Animal Science and Technology, Northwest Agriculture &4. Forest University, Yangling, Shanxi, PR China |
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| Abstract: | A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed using a new monoclonal antibody for detecting the food colourant Sudan I. The half-maximum inhibition concentrations (IC50) and the limit of detection (calculated as IC20) of ELISA for Sudan I were 2 and 0.01 ng mL?1, respectively. The study showed little cross-activity with Sudan I structural analogues (below 0.01%). The average recoveries in intra- and interassays for Sudan I from fortified fresh tomato and chilli samples by ELISA were in ranges of 82–94% and 79–91%, respectively. The coefficients of variation of intra- and interassays were 6–8% and 6–10%, respectively. The IC50 was approximately 2.0 ng mL?1 after the Sudan I-Kit had been kept for 180 days. |
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| Keywords: | Sudan I monoclonal antibody ELISA fresh tomato chilli |
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