| 蜈蚣三七有效成分W3对小鼠脾淋巴细胞增殖的影响 |
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| 引用本文： | 邴飞虹,张国斌,苏彦奇. 蜈蚣三七有效成分W3对小鼠脾淋巴细胞增殖的影响[J]. 中国药师, 2010, 13(7): 932-935 |
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| 作者姓名： | 邴飞虹 张国斌 苏彦奇 |
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| 作者单位： | 湖北中医药大学药学院,武汉,430065 |
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| 摘 要： | 目的：研究林荫银莲花根茎蜈蚣三七中所含有效成分W3对小鼠脾淋巴细胞增殖的影响.方法：利用硅胶柱和C18色谱柱分离纯化W3,采用四甲基偶氮唑蓝法分别检测蜈蚣三七总皂苷、W3及其苷元齐墩果酸对小鼠脾淋巴细胞体外增殖反应的影响.结果：W3对正常增殖的淋巴细胞活性无明显影响;剂量≥0.1μmol·L-1时,W3对刀豆蛋白诱导的淋巴细胞增殖活性有显著的抑制作用（P＜0.05）.结论：蜈蚣三七有效成分W3具有较强的抑制淋巴细胞增殖活性.
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| 关 键 词： | 蜈蚣三七 淋巴细胞 增殖 |
Effects of W3 Extracted from Anemone flaccida on Spleen Lymphocyte Proliferation in Mice |
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| Bing Fei hong,Zhang Guobin,Su Yanqi. Effects of W3 Extracted from Anemone flaccida on Spleen Lymphocyte Proliferation in Mice[J]. China Pharmacist, 2010, 13(7): 932-935 |
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| Authors: | Bing Fei hong Zhang Guobin Su Yanqi |
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| Affiliation: | Bing Feihong,Zhang Guobin,Su Yanqi(College of Pharmacy,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China) |
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| Abstract: | Objective： To investigate whether W3, the chief component from rhizome （named Wugong Sanqi） of Anemone flaccida, has immunoaetivity. Method： W3 was isolated and purified by solvent extractions, silica gel column and CIs reversed-phase column. Effects of Wugong Sanqi extract, W3 and oleanolic acid （ mother nucleus of W3 ） on the mouse spleen lymphocyte proliferation in vitro were determined by a MTTassay. Result： W3 （ ≥0.1μmol·L-1 ） significantly inhibited the proliferation of lymphocytes induced by ConA （P 〈 0.05） while no obvious influences on lymphocyte proliferation were observed in oleanolie acid group. Condusion：sW3 hows a good immunoactivity. |
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| Keywords: | Anemone flaccida Lymphocyte Proliferation |
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