Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha,India |
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Authors: | Biswaprakash Pradhan Sashi K Dash Sabuj Sahoo |
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Affiliation: | 1.Pharmaceutical Biotechnology Division, University Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha-751 004, India.;2.Department of Seed Technology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751 003, India.;REQUIMTE/FFUP (Portugal), Tel: +351 220 428 500, ext. 8856, E-mail: , |
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Abstract: | ObjectiveTo screen and isolate an eco-friendly, a thermophilic and potent L-asparaginase producing bacterium, with novel immunological properties that may obviates hypersensitivity reactions.MethodsIn the present study bacterial strain isolated for extracellular L-asparaginase production from hotspring, identified by morphological, biochemical and physiological tests followed by 16S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.ResultThe bacterial strain was identified as Bacillus subtilis strain hswx88 (GenBank Accession Number: {"type":"entrez-nucleotide","attrs":{"text":"JQ237656.1","term_id":"374341559","term_text":"JQ237656.1"}}JQ237656.1). The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88 (23.8 IU/mL) was found to be 1.7 and 14.5 times higher than the reference organism Pectobacterium carotovorum MTCC 1428 (14.2 IU/mL) and Bacillus sp. BCCS 034 (1.64 IU/mL).ConclusionThe isolate is eco-friendly and useful to produce bulk quantity of extracellular, thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation. |
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Keywords: | L-asparaginase Thermophilic bacteria Bacillus subtilis strain hswx88 16S rDNA. |
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