3种药膳对小鼠抗疲劳的效果

目的探讨3种药膳制剂对小鼠的抗疲劳作用,为研究抗疲劳制剂提供实验依据。方法将120只雄性昆明种小鼠按体质量随机分为4组,分别为参蚁药酒组、药膳Ⅰ号膏剂组、药膳Ⅱ号膏剂组和对照组;每组30只,分批实验,每次实验从对应组中随机抽取10只小鼠。灌喂实验制剂15d后,分别检测小鼠体质量、力竭游泳时间和血乳酸、血清尿素氮、肝糖原、肌糖原。结果参蚁药酒组、药膳Ⅰ号、Ⅱ号膏剂组和对照组小鼠的力竭游泳时间分别为（411.0±22.2）s、（325.0±31.2）s、（595.0±53.9）s、（231.0±22.5）s,3个药膳组均明显长于对照组（P〈0.05,P〈0.01）;各组运动后肝糖原含量分别为（15.7±4.7）mg/g、（23.1±6.1）mg/g、（32.0±5.4）mg/g、（12.3±2.5）mg/g,3个药膳组均明显高于对照组（P〈0.05,P〈0.01）;各组运动后血清尿素氮含量分别为（9.5±1.1）mmol/L、（9.9±0.6）mmol/L、（10.0±1.1）mmol/L、（11.8±1.5）mmol/L,3个药膳组均明显低于对照组（P〈0.01）;Ⅰ、Ⅱ号膏剂组运动后血乳酸的生成量均低于对照组。几项检测指标中,Ⅱ号膏剂组与对照组比较差异最明显（P〈0.01）。结论3种药膳制剂对小鼠均有明显的抗疲劳效果,且Ⅱ号药膳膏剂效果最显著。

ANTI-FATIGUE EFFECT OF THREE CHINESE MEDICINE PREPARATIONS ON MICE

Objective To explore the anti-fatigue effect of Chinese medicine preparations on mice, so as to provide experimental basis for studying anti-fatigue substance. Methods One hundred and twenty mice were randomly divided into control group and 3 experimental groups. Ginseng-ant medicinal liquor（GAL）, No. 1 medicine-diet, No. Ⅱ medicine-diet were given to the 3 experimental groups respectively, while distilled water to the control group for 15 d. The swimming time, blood lactic acid, serum urea nitrogen（SUN）, hepatic glycogen, and muscle glycogen were assayed, Results The swimming time of GAL group, No. Ⅰ medicine-diet group, and No. Ⅱ medicine-diet group（411.0 ± 222）s, （325.0± 312）s, （595.0 ± 539）s respectively] was significantly longer than that of control group （231.0 ± 225） s, P 〈 0.05, P 〈 0.011. The hepatic glycogen content of 3 experimental groups （15.68 ±4.67）mg/g, （23.14±6.12）mg/g, （31.99± 5.37）mg/g respectively3 was higher than that of control group （ 12.27 ± 2.5） rag/g, P 〈 0.05, P 〈 0.01 ]. The SUN content of the 3 experimental groups （9.5 ± 1.1）mmol/L, （9.9 ± 0.6）mmol/L, （10±1.1 ））Inmol/L respectively] was less than that of the control group （ 11.8 ± 1.5） mmol/L, （ P 〈 0.01 ） 1. Moreover, less accumulation of lactate content in 2 medicine-diet groups was found. In general, No 11 medicine-diet was more efficacious than others. Condusion All 3 Chinese medicine preparations, especially No Ⅱ medicine-diet, have anti-fatigue effect on mice.