Involvement of the Pta-AckA pathway in protein folding and aggregation |
| |
| Authors: | Itzhak Mizrahi Dvora Biran Eliora Z. Ron |
| |
| Affiliation: | 1. Laboratoire VEAC, Université Paris-Sud 11, Faculté des Sciences, Bât. 360, Rue du Doyen André Guinier, 91405 Orsay, France;2. Pôle Risques MRSH-CNRS, EA2608, Université de Caen, Esplanade de la Paix, 14032 Caen, France;3. IFR141-IPSIT, Plateforme d’Imagerie Cellulaire, Université Paris-Sud 11, Faculté de Pharmacie, Tour E1, 5 Rue Jean-Baptiste Clément, 92296 Châtenay-Malabry, France;4. Service de Biologie Cellulaire, Université Paris-Sud 11, Faculté des Sciences, Bât. 336, Rue du Doyen André Guinier, 91405 Orsay, France;3. Departments of Microbiology, Urbana, Illinois 61801 and;4. Departments of Biochemistry, University of Illinois, Urbana, Illinois 61801;1. State Key Laboratory of Particle Detection and Electronics, University of Science and Technology of China, No. 96, Jinzhai Road Hefei 230026, People''s Republic of China;2. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, People''s Republic of China;1. Centre for Clean Environment and Energy, Griffith University, Gold Coast Campus, QLD 4222, Australia;2. College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China;3. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;4. School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China;1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;2. Center for Systems Biology, University of Iceland, Reykjavik, Iceland |
| |
| Abstract: | Acetyl phosphate is a central metabolite involved in a broad range of versatile cellular functions. Recently it was observed that in Escherichia coli the acetyl phosphate pathway is required for efficient ATP-dependent proteolysis. Deletion of the operon coding for acetyl phosphate metabolism (ΔackApta) results in a very low cytoplasmic level of acetyl phosphate and impaired proteolysis. Here we show that the ΔackApta mutation affects additional components of the protein quality control system. Thus, this deletion is accompanied by a decrease in protein refolding and rescue from aggregates. These results indicate the involvement of the acetyl phosphate pathway in chaperone capabilities, in addition to their effect on proteolysis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
