Terminal-restriction fragment length polymorphism analysis of biphenyl dioxygenase genes from a polychlorinated biphenyl-polluted soil |
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| Authors: | Serena Capodicasa Stefano Fedi Monica Carnevali Leonardo Caporali Carlo Viti Fabio Fava Davide Zannoni |
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| Affiliation: | 1. Department of Experimental Evolutionary Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;2. Department of Agricultural Biotechnology, University of Firenze, Piazzale delle Cascine 24, 50144 Firenze, Italy;3. Department of Applied Chemistry and Material Science, University of Bologna, Via Terracini 28, 40131 Bologna, Italy;1. Department of Chemical, Environmental and Materials Engineering, University of Jaén, Campus ‘Las Lagunillas’, 23071 Jaén, Spain;2. Department of Chemical Engineering, University of Granada, Campus ‘Fuentenueva’, 18071 Granada, Spain;1. Department of Urology, University Hospital Mainz, Mainz, Germany;2. Department of Urology, Hospital Berlin-Buch, Berlin, Germany;3. Department of Urology, University Hospital Hamburg-Eppendorf, Hamburg, Germany;4. Department of Urology, University Hospital Regensburg, Regensburg, Germany;5. Department of Urology, Hospital Garmisch-Patenkirchen, Garmisch-Patenkirchen, Germany;6. Department of Urology, Federal Armed Services Central Hospital Koblenz, Koblenz, Germany;7. Department of Urology, University Hospital Lubeck, Lubeck, Germany;8. Department of Urology, University Hospital Cologne, Cologne, Germany;1. Forest & Landscape, Faculty of Science, University of Copenhagen, Rolighedsvej 23, DK-1958 Frederiksberg C, Copenhagen, Denmark;2. Plant and Soil Science Section, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark;1. ICV-GVM la Roseraie, 120, avenue de la République, 93300 Aubervilliers, France;2. ICPS, 6, avenue du Noyer-Lambert, 91300 Massy, France;3. Institut Claude-Gallien, 20, route de Boussy, 91480 Quincy-sous-Sénart, France |
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| Abstract: | Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that can be co-metabolically biotransformed by biphenyl-utilizing bacteria. In this study, terminal-restriction fragment length polymorphism (T-RFLP) was applied to the substrate specificity-determining region of the 2,3-biphenyl dioxygenase encoding genes of a microbial community found in a PCB-polluted soil. Notably, both the total biphenyl/PCB-utilizing community and its members actively expressing the 2,3-biphenyl dioxygenase gene were analyzed. T-RFLP fingerprinting along with gene library construction allowed us not only to detect biphenyl dioxygenases related to the well characterized catabolic patterns of Pseudomonas pseudoalcaligenes KF707 and Burkholderia xenovorans LB400, but also to reveal novel environmental enzyme classes displaying amino acid substitutions that may be related to broader specificity and improved catalytic properties. Furthermore, space and time of sampling along with bioavailability conditions of different PCBs were considered possible sources of profile variability. |
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