Quantitative PCR of pmoA using a novel reverse primer correlates with potential methane oxidation in Finnish fen |
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| Authors: | Tero T. Tuomivirta Kim Yrj?l? Hannu Fritze |
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| Affiliation: | 1. Finnish Forest Research Institute, Vantaa Research Unit, Box 18, FI-01301 Vantaa, Finland;2. Department of Biosciences, University of Helsinki, PO Box 56, FI-00014 Finland;1. Department of Environmental Engineering, Zhejiang University, Hangzhou, China;2. College of Environment, Zhejiang University of Technology, Hangzhou, China;3. Department of Chemistry, University of Science & Technology of China, Hefei, China;4. Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Nijmegen, the Netherlands;5. Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, Hangzhou, China;1. Faculty of Environmental Design, Institute of Science and Engineering, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1192, Japan;2. Department of Environmental System Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan;3. Department of Science of Technology Innovation, Nagaoka University of Technology, Kamitomioka 1603-1, Nagaoka, Niigata 940-2188, Japan;4. Department of Social and Environmental Engineering, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, Hiroshima 739-8527, Japan |
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| Abstract: | We report a new reverse primer (A621r) for use with A189f in PCR amplification of pmoA alleles in type II methanotrophs. The new primer combination was used to successfully amplify pmoA in peat monolith samples of various depths taken from fen-type peatlands in Finland. In quantitative PCR, pmoA amplicons produced from two sets of three replicate monoliths showed a significant Pearson correlation coefficient (r = 0.77 and 0.61) with methane oxidation potential. The maximum methane oxidation potential and number of pmoA amplicons ranged between 8.8–40.5 μmol g (dry weight)?1 d?1 and 5.5 × 107–18.7 × 107 g (wet weight)?1, respectively, occurring in depths between 10 and 30 cm beneath the surface in the seven individual monoliths used in this study. |
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