Retinal gliopathy accompanying thioacetamide-induced liver insufficiency: light and electron microscopic observations |
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Authors: | J Albrecht Roman Gadamski Heidrun Kuhrt Micha Walski A Reichenbach |
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Institution: | (1) Medical Research Center, Polish Academy of Sciences, Warsaw, Poland, PL;(2) Department of Neurophysiology, Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, D-04109 Leipzig, Germany Tel.: 49-341-9725731; Fax: 49-341-9725739; e-mail: reia@server3.medizin.uni-leipzig.de, DE |
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Abstract: | A recent examination of retinae of patients who had died with symptoms of liver insufficiency (LI) including hepatic encephalopathy
(HE) revealed morphological changes in retinal Müller glia similar to the astrocytic changes normally accompanying HE, and
the term “hepatic retinopathy” (HR) was coined to define these changes. In the present study, the immunomorphology and ultrastructure
of Müller cells were examined in rats in which LI with accompanying HE was induced with a hepatotoxin, thioacetamide (TAA).
Light microscopically, retinae of rats with LI were characterized by swelling of the Müller cell cytoplasm. Immunostaining
for glia-specific marker proteins in Müller cells from LI rats revealed a strongly enhanced expression of glial fibrillary
acidic protein, and a considerable increase in glutamine synthetase immunoreactivity, as compared to control animals. Ultrastructurally,
the Müller cells of LI rats showed swelling and vacuolization of cell processes. In particular, the endfeet contained many
swollen mitochondria. By contrast, LI produced no morphologically demonstrable changes in retinal neurons and photoreceptor
cells. Thus, the retinal changes induced by TAA in the rats strongly resembled those described in human HR, rendering the
present rat model suitable for more detailed investigations of the pathomechanism(s) of HR.
Received: 4 August 1997 / Revised, accepted: 12 January 1998 |
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Keywords: | Rat Thioacetamide Liver insufficiency Hepatic encephalopathy Müller glia |
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