哮喘儿童IL-13水平和肺功能1秒钟用力呼气容积变化的研究

目的:观察哮喘急性发作期儿童经GINA方案的系统治疗前后外周血单个核细胞(PBMC) 培养上清液中IL-13和肺功能1秒钟用力呼气容积(FEV1 ) 的变化及相互关系。方法: 采用双抗体夹心ELISA法测定23例中度急性发作期哮喘患儿治疗前后、正常对照组儿童(20例) PBMC培养上清液中IL-13水平, 并同时测肺功能1秒钟用力呼气容积(FEV1 ) 占预计值的百分比。结果: 哮喘患儿急性发作期PBMC培养上清液中IL-13水平( 169. 66±14. 51 ) ng/L较正常对照组( 92 .68±13. 80) ng/L为高, 差别非常显著(P<0 .01)。同时显示, 哮喘患儿急性发作期FEV1 值占预计值的百分比为(69. 78±8 .30),明显低于正常对照组(98 .35±5 .36), 差别非常显著(P<0 .01)。哮喘患儿治疗前、后PBMC培养上清液中IL-13测定: 治疗后为(120 .21±16 .35) ng/L明显低于急性发作期, 仍高于正常对照组, 差别非常显著(P<0 .01)。FEV1 占预计值的百分比测定: 治疗后为(82 .56±10. 40) 明显高于急性发作期, 肺功能有所改善, 但仍低于正常对照组, 差别非常显著(P<0. 01)。直线相关分析表明, 哮喘急性发作期IL-13与FEV1 占预计值的百分比呈负相关(r=-0 493, P<0 05)。结论: IL-13在哮喘的急性发病中起着重要作用, 其可能是构成气道慢性炎症的各类因素之一。IL-13水?

Study on changes of IL - 13 level and lung ventilation function forced expiratory volume in one second in children with asthma

Objective:To observe the changes of IL-13, lung ventilation function forced expiratory volume in one second (FEV1) and their correlation before and after anti-asthmatic treatment for mild asthmatic children with acute asthma attack.Methods:The levels of IL-13 in the supernatant of cultured peripheral blood mononuclear cells (PBMC) were separately assessed with double-antibody sandwich ELISA in 23 mild asthmatic children with acute asthma attack before and after anti-asthmatic treatment, and 20 healthy volunteers. Lung ventilation function FEV1 was determined in all patients. Results:The level of IL-13〔(169.66±14.51)ng/L〕 in mild asthmatic children with acute attack was significantly higher than that〔(92.68±13.80)ng/L〕in normal control group( P <0.01). As the same time, the percentage of FEV1 accounting for predictive(FEV1%Pred) value(69.78±8.30) in mild asthmatic children with acute asthma attack was significantly higher than that (98.35±5.36)in normal control group( P <0.01). After anti-asthmatic treatment, the level of IL-13〔 (120.21±16.35)ng/L〕was obviously lower than that before treatment, but the level of IL-13 was significantly higher than that in normal control group ( P <0.01). The value of FEV1%Pred (82.56±10.40) was higher than that before treatment, the lung ventilation function improved, but the value of FEV1%Pred was significantly lower than that in normal control group( P <0.01).Conclusion:analysis showed that the level of IL-13 was negatively correlated to FEV1 ( r=-0.493, P <0.05). Conclusion: IL-13 should play an important role in asthmatic attack, which might be one of factors resulting in bronchial chronic inflammation. The study on changes of IL-13 level and FEV1 in asthmatic children should have an important value for asthmatic diagnosis and judgment of curative effect, which could supply a beneficial basis for IL-13 receptor antagonist as a new anti-inflammation drugs.