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| 蛋白质组学方法鉴定卵巢癌转移相关蛋白 | |
| 作者姓名： | Zhou L Yang XF Wang Y Zhang YT Geng YP Si LS Wang YL |
| 作者单位： | 1. 西安交通大学第二医院神经外科 2. 西安交通大学第一医院妇产科 3. 西安交通大学癌症研究所,西安交通大学生物医学信息工程教育部重点实验室,710061 |
| 摘 要： | 目的探讨不同转移潜能卵巢癌细胞株蛋白表达谱差异。方法采用基于双向电泳（2-DE）-基质辅助激光解析电离飞行时间质谱（MALDI—TOF—MS）蛋白质组学分析方法,对比分析具有相同来源、不同转移能力的卵巢癌细胞系HO8910和HOg910pm的总蛋白表达谱差异。结果2-DE分析发现两株细胞间有39个蛋白表达水平差异在2倍以上,MALDI—TOF—MS鉴定出其中18种蛋白,它们涉及细胞凋亡、细胞外基质、细胞骨架、生长因子、糖酵解、蛋白质代谢和免疫系统等领域。结论肿瘤转移涉及体内多种领域多种蛋白,这些领域的蛋白相互协调作用最终共同控制细胞的转移能力。 |
| 关 键 词： | 卵巢肿瘤 肿瘤转移 蛋白质组学 |
Identification of metastasis-associated proteins of ovarian cancer by proteomics |
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| Zhou L,Yang XF,Wang Y,Zhang YT,Geng YP,Si LS,Wang YL.Identification of metastasis-associated proteins of ovarian cancer by proteomics[J].Chinese Journal of Pathology,2007,36(12):814-818. | |
| Authors: | Zhou Le Yang Xiao-Feng Wang Yan Zhang Ying-Tao Geng Yi-Ping Si Lü-Sheng Wang Yi-Li |
| Institution: | The Key Laboratory of Biomedical Information Engineering of National Administration of Education, Institute for Cancer Research, Xi'an Jiaotong University, Xi'an, China. |
| Abstract: | OBJECTIVE: To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities. METHODS: The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach. RESULTS: Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system. CONCLUSION: The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer. |
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