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| 血管内皮生长因子反义RNA对体外培养的人脑垂体瘤细胞增殖和凋亡的影响 | |
| 引用本文： | 王建祯,付瑞锋,宋乐,臧卫东,牛光明,娄季宇,孙二平.血管内皮生长因子反义RNA对体外培养的人脑垂体瘤细胞增殖和凋亡的影响[J].中国实用神经疾病杂志,2001,4(5):3-4. |
| 作者姓名： | 王建祯 付瑞锋 宋乐 臧卫东 牛光明 娄季宇 孙二平 |
| 作者单位： | 1. 郑州大学第二附属医院神经科,郑州,450003 2. 中原油田总医院第二分院外科,濮阳,457000 3. 郑州大学医学院人体解剖教研室,郑州,450052 4. 河南省西平县人民医院,462100 |
| 摘 要： | 目的 :研究血管内皮生长因子 (VEGF)反义RNA对体外培养人脑垂体瘤细胞增殖和凋亡的影响。方法 :将VEGF反义cDNA(反义组 )、正义VECFcDNA质粒 (正义组 )转染垂体瘤细胞 ;应用免疫组化法和TUNEL法分别检测肿瘤细胞增殖指数及细胞凋亡。结果 :反义组细胞增殖指数及细胞凋亡数量与正义组、未转染组相似 ,差异无显著性 (P <0 0 5)。结论 :VEGF反义RNA对体外培养人脑垂体瘤细胞增殖和凋亡无明显影响 ,为垂体瘤基因治疗基础研究提供依据 |
| 关 键 词： | 血管内皮生长因子反义RNA 垂体瘤 增殖 凋亡 |
| 修稿时间： | 2001年6月21日 |
The effect of VEGF antisense RNA on the proliferation activity and apoptosis of human pituitary adenoma cells in vitro |
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| Wang Jianzhen,Fu Ruifeng,Song Le,et al..The effect of VEGF antisense RNA on the proliferation activity and apoptosis of human pituitary adenoma cells in vitro[J].Chinese Journal of Practical Neruous Diseases,2001,4(5):3-4. | |
| Authors: | Wang Jianzhen Fu Ruifeng Song Le |
| Institution: | Wang Jianzhen,Fu Ruifeng,Song Le,et al.Department of Neurology,the second affiliated hospital of Zhengzhou University,Zhengzhou 450003 |
| Abstract: | Objective:To study the effect of VEGF antisense RNA on the proliferation activity and apoptosis of human pituitary adenoma cells in vitro.Methods:Carrying with VEGF antisense cDNA,sense VEGF cDNA plasmid were transfected into pituitary adenoma cells,the proliferation activity and apoptosis were detected by immunohistochemistry and TUNEL method.Results:There was no significant difference of the proliferation activity and apoptosis between the antisense group,sense group and control group (P>0 05).Conclusion:VEGF had no effect on the cell proliferation and induction of cell apoptosis,and provided evidence for gene therapy of pituitary adenoma. |
| Keywords: | VEGF antisense RNA Human pituitary adenoma Proliferation activity Apoptosis |
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