抑癌基因PTEN在鼻咽癌中的表达及突变

目的了解抑癌基因PTEN在鼻咽癌中的表达、突变情况,探讨其与鼻咽癌发生发展的关系。方法采用免疫组化检测52例鼻咽癌中PTEN基因的表达,利用PCR-SSCP银染、DNA测序分析等方法检测PTEN基因第5、8外显子突变。结果鼻咽癌中PTEN蛋白表达率低于癌旁组织,表达与临床TNM分期、颈部淋巴结转移密切相关（P〈0.05）。52例鼻咽癌中PTEN基因第5、8外显子未见明显突变。结论抑癌基因PTEN可能参与鼻咽癌的发生发展,其第5、8外显子在鼻咽癌的发生发展中可能不起关键作用。

Expression and mutation analysis of tumor suppressor PTEN in nasopharyngeal carcinoma

Objective To detect the expression and mutation of tumor suppressor PTEN in nasopharyngeal carcinoma(NPC),and to investigate the relationship of the PTEN gene to pathogenesis and development of NPC.Methods Expression levels of PTEN in 52 cases of NPC were determined by an immunohistochemistry method,and polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP), while silver staining and DNA sequencing were used to detect the mutation in exon 5 and 8 of the PTEN gene in NPC.Results Differences of the PTEN expression were significant between NPC and nasopharyngitis(P<0.05).Expressions of PTEN were closely related to lymph node metastasis and clinical TNM stage(P<0.05).None of 52 cases showed mutation in exon5 and 8 of the PTEN gene in NPC.Conclusion PTEN may have some effects on the pathogenesis and development of NPC,and mutation in exon 5 and 8 of PTEN may not play a main role in the genesis and development of NPC.