miR-361-5p对肾细胞癌ACHN细胞恶性生物学行为的影响

目的：探讨miR-361-5p对肾细胞癌ACHN细胞增殖、侵袭、迁移、凋亡及其细胞周期的影响。方法：将miR-361-5p mimics和miR-361-5p inhibitor分别转染至肾癌ACHN细胞中，用qPCR检测转染细胞中miR-361-5p的表达水平，用MTT法、划痕愈合实验、Transwell实验、流式细胞术分别检测细胞的增殖、迁移、侵袭、细胞周期和凋亡水平。结果：与空白对照组和 Mimics-NC组比较，miR-361-5p mimics组ACHN细胞中miR-361-5p表达水平显著升高（P<0.01），细胞的增殖、侵袭和迁移能力均显著减弱（均P<0.01），而凋亡率升高（P<0.01）。与空白对照组或Inhibitor-NC组比较，miR-361-5p inhibitor组细胞中miR-361-5p表达水平显著下降（P<0.01），细胞的增殖、侵袭和迁移能力均增强（均P<0.01），细胞周期运转加速（P<0.01），凋亡率降低（P<0.05）。结论：miR-361-5p可抑制肾癌ACHN细胞的增殖、侵袭和迁移，并诱导细胞凋亡，其在肾癌发生发展过程中发挥重要的抑制作用。

Effects of miR-361-5p on malignant biological behaviors of renal cell carcinoma ACHN cells

Objective: To investigate the effect of miR-361-5p on proliferation, invasion, migration, apoptosis and cell cycle of renal cell carcinoma ACHN cells. Methods: MiR-361-5p mimics and miR-361-5p inhibitor were transfected into ACHN cells, respectively.The expression of miR-361-5p in the transfected cells was detected by Real-time quantitatibse PCR; and the proliferation, migration,invasion, cell cycle and apoptosis of cells were detected by MTT assay, Scratch-healing assay, Transwell assay and flow cytometry,respectively. Results: Compared with the control group and mimics-NC group, the expression of miR-361-5p was increased significantly in ACHN cells of -miR-361-5p mimics group (P<0.01), the abilities of cell proliferation, invasion and migration were decreased significantly (all P<0.01), and the apoptosis rate was increased (P<0.01). Compared with the control group or inhibitor-NC group, the expression of miR-361-5p was significantly decreased in ACHN cells of miR-361-5p inhibitor group (P<0.01), the abilities of cell proliferation, invasion and migration were increased (all P<0.01), the cell cycle was accelerated (P<0.01), and the apoptosis rate was decreased (P<0.05). Conclusion: miR-361-5p can inhibit the proliferation, invasion and migration of renal cell carcinoma ACHN cells,and induce cell apoptosis, which plays an important inhibitory role in the development of renal cell carcinoma.