巨噬细胞移动抑制因子在不同分化程度胃腺癌组织中的表达及临床意义

目的 检测巨噬细胞移动抑制因子(MIF)在不同分化程度胃腺癌组织、癌旁组织及正常胃黏膜组织中的表达,分析MIF的表达与胃腺癌分化程度、淋巴结转移情况及其他临床病理特征相关性.方法 选择胃腺癌组织120例及癌旁组织40例为实验组,其中高、中、低分化腺癌各40例,选择正常胃黏膜组织40例为对照组.应用免疫组化法检测不同分化程度胃腺癌组织、癌旁组织、正常胃黏膜组织中MIF表达情况.结果 胃腺癌MIF表达阳性率为80.8%(97/120),低分化、中分化、高分化胃腺癌MIF表达阳性率分别为97.5%(39/40),82.5%(33/40),62.5%(25/40).癌旁组织MIF表达阳性率为40%(16/40),正常胃黏膜MIF表达阳性率为7.5%(3/40).低、中、高分化胃腺癌MIF表达阳性率Spearman等级相关分析系数rs为0.458(x2=27.046,P＜0.001).MIF的表达与临床病理因素关系相关性为淋巴结转移x2=9.231(P=0.002)、性别x2=0.045(P=0.832)、年龄为x2=0.241(P=0.623)、肿瘤大小x2=0.003(P=0.954)、肿瘤部位x2=0.051(P=0.821).结论 胃腺癌组织MIF表达明显高于癌旁组织及正常胃黏膜组织MIF表达,低分化腺癌MIF表达明显高于高/中分化腺癌MIF表达.MIF表达与胃腺癌组织分化程度及淋巴是否转移密切相关,与年龄、性别、肿瘤大小、肿瘤部位无相关性.

Expression and significance of macrophage migration inhibitory factor in gastric adenocarcinoma

Objective To investigate the expression of MIF on the gastric adenocarcinoma, evaluate the relation between the MIF expression and tumor differentiation, lymph node metastasis and other clinical patho-features. Methods 120 gastric adenocarcinoma cancer tissues, 40 tissues besides cancer and 40common mucosa tissues specimens are collected. Detect the expression of MIF with immunohistochemistry method (Streptavidin -Peroxidase). Results Positive rate of MIF expression in gastric cancer is 80. 8%(97/120). Those in poorly differentiated, moderately differentiated and well-differentiated gastric adenocarcinoma are 97. 5% (39/40), 82. 5% (33/40) ,62.5% (25/40), analytical factor of Spearman rank correlation rs is 0. 458( x2 =27. 046, P ＜0.001 ). Positive rate of MIF expression in tissues besides cancer is 40% (16/40)and that in normal gastric mucosa is 7.5% (3/40). Positive rate of MIF expression in gastric adenocarcinoma without lymph node metastasis is 40% (22/55) and the rate with lymph node metastasis is 67% (44/65). Conclusions There is overexpression of MIF in the gastric adenocarcinoma.Expression of MIF in different differentiated cancer is different. Expression in poorly differentiated is higher than that in moderately differentiated and well-differentiated caner. The level of expression of MIF malignancy has positive correlation with malignancy degree of gastric adenocarcinoma. MIF expression has nothing to do with age, gender, tumor sizes or location.