Ph+和Ph-CML病人外周血TCR Vβ T细胞分布特点

为了解Ph^ 和Ph^-慢性粒细胞白血病（CML）病人外周血TCR Vβ亚家族T细胞的分布特点，利用RT－PCR分别扩增13例CML病人（Ph^ -b3a2型5例，Ph^ -b2a2型5例，Ph^-型3例）外周血单个核细胞的TCR Vβ的24个亚家族基因片段，了解病人各Vβ亚家族的利用情况。研究结果：发现与正常人有所不同，病人仅存在部分TCR Vβ亚家族T细胞，Ph^ -b3a2型CML表达4－16（平均10．2）个Vβ亚家族，Ph^ -b2a2型CML表达8－11（平均8．8）个Vβ亚家族，而Ph^-者表达506（平均5．7）个Vβ亚家族。各病人表达的Vβ亚家族分布有所不同，在Ph^ (b3a2型）全部病人表达Vβ10和Vβ16，其次为Vβ9和Vβ22；Ph^ （b2a2型）除与Ph^ (b3a2型）相同全部病人表达Vβ10和Vβ16外，还全部表达Vβ24，其次为Vβ8；而Ph^-者全部表达Vβ24，其次为Vβ3，Vβ10，Vβ13和Vβ22。结论：提示Ph^ 和Ph^-CML病人外周血的TCR Vβ亚家族T细胞存在倾斜性分布现象，不同类型CML病人T细胞的TCR Vβ选择性利用有所不同，这可能与CML细胞相关抗原的差异以及个体特异性免疫反应的差异有关。

The Distribution Feature of TCR Vβ Repertoire in Peripheral Blood T Cells from Patients with Ph + and Ph- CML

To investigate the T cell distribution characters of TCR Vbeta repertoire in Ph(+) and Ph(-) CML. The 24 subfamilies of the TCR Vbeta genes were amplified in peripheral blood T cells from 13 patients with CML (Ph+ b3a2, 5 cases; Ph+ b2a2, 5 cases; Ph(-), 3 cases) by RT-PCR, to analyze the usage of Vbeta subfamilies in different CML patients. The results showed that the expression pattern of Vbeta repertoire was different in normal individuals and in patients with CML which only have part of Vbeta subfamily T cells. 4 - 16 (mean 10.2) Vbeta subfamily T cells were detected in the Ph+ b3a2 CML, 8 - 11 (mean 8.8) Vbeta subfamily T cells in the Ph(+) b2a2 CML and 5 - 6 (mean 5.7) in Ph(-) CML. Moreover, the expression of Vbeta subfamily T cells was different among these three types CML.Vbeta10 and Vbeta16 were detected in the all cases with Ph(+) b3a2 and Ph(+) b2a2 CML, whereas Vbeta9 and Vbeta22 could be found in the most cases with Ph(+) b3a2 CML or Vbeta24 and Vbeta8 in Ph(+) b2a2 CML. In patients with Ph(-) CML, Vbeta24 were detected in all samples, and Vbeta9, Vbeta10, Vbeta13 and Vbeta22 were found in the most cases. The results suggest that skew distribution of TCR Vbeta subfamily T cells was existed in peripheral blood of Ph(+) and Ph(-) CML patients. The selected usage of TCR Vbeta is different in various types of CML patients. It may relate to difference of CML cells associated antigen and individual special immunity reaction.