| 高效液相色谱法测定银花感冒冲剂中绿原酸含量 |
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| 引用本文： | 李建芳,黄劲通.高效液相色谱法测定银花感冒冲剂中绿原酸含量[J].中国药业,2013,22(14):63-64. |
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| 作者姓名： | 李建芳 黄劲通 |
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| 作者单位： | 1. 广西壮族自治区北海食品药品检验所,广西北海,536000
2. 广西恒拓集团仁盛制药有限公司,广西防城港,535500 |
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| 摘 要： | 目的建立银花感冒冲剂中绿原酸含量测定的高效液相色谱法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸溶液(13∶37),流速为1.0 mL/min,检测波长为327 nm。结果绿原酸进样量在0.050 4～1.008 0μg范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.33%,RSD为0.61%(n=6)。结论该方法简便、结果准确、重现性好,可作为银花感冒冲剂中绿原酸的含量测定方法。
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| 关 键 词： | 银花感冒冲剂 绿原酸 高效液相色谱法 含量测定 |
Determination of Chlorogenic Acid Content in Yinhuaganmao Electuary by HPLC |
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| Li Jianfang
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Huang Jingtong.Determination of Chlorogenic Acid Content in Yinhuaganmao Electuary by HPLC[J].China Pharmaceuticals,2013,22(14):63-64. |
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| Authors: | Li Jianfang Huang Jingtong |
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| Institution: | 1.Beihai Municipal Institute for Food and Drug Control,Beihai,Guangxi,China 536000; 2.Rensheng Pharmaceutical Co.,LTD.,Guangxi Hengtuo Group,Fangchenggang,Guangxi,China 535500) |
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| Abstract: | Objective To establish an HPLC method for determining the oleanolic acid content in Yinhuaganmao Electuary by HPLC.Methods The Kromasil C 18 column（ 250 mm × 4.6 mm,5 μm） was adopted and the mobile phase consisted of acetonitrile0.4% phosphoric acid（ 13 ∶ 37） with the flow rate of 1.0 mL / min.The detecting wavelength was 327 nm.Results The sample size of chlorogenic in the range of 0.050 4-1.008 0 μg（ r = 0.999 9） showed the good linear relation with the peak area,the average recovery rate was 99.33%,RSD was 0.61%.Conclusion This methods is simple with accurate results and good reproducibility,and can be used to the content determination method of chlorogenic acid in Yinhuaganmao Electuary. |
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| Keywords: | Yinhuaganmao Electuary chlorogenic acid HPLC contents determination |
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