| 高效液相色谱-蒸发光散射器法测定川贝末胶囊中贝母素乙含量 |
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| 引用本文： | 汪剑飞.高效液相色谱-蒸发光散射器法测定川贝末胶囊中贝母素乙含量[J].中国药业,2013,22(14):53-55. |
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| 作者姓名： | 汪剑飞 |
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| 作者单位： | 安徽省芜湖市食品药品检验所,安徽芜湖,241001 |
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| 摘 要： | 目的建立测定川贝末胶囊中贝母素乙含量的高效液相色谱-蒸发光散射器(HPLC-ELSD)法。方法采用IntersiL C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%二乙胺(70∶30)为流动相,流速为1.0 mL/min,蒸发光散射检测器检测。结果贝母素乙进样质量的线性范围为0.6～6.0μg(r=0.999 5),平均加样回收率为100.16%,RSD为1.69%(n=6)。结论该法简便快速、结果准确可靠,能准确测定川贝末胶囊中贝母素乙的含量,可用于该制剂的质量控制。
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| 关 键 词： | 高效液相色谱-蒸发光散射器法 川贝末胶囊 贝母素乙 |
Determination of Peiminine in Chuanbeimo Capsule by HPLC-ELSD |
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| Wang Jianfei.Determination of Peiminine in Chuanbeimo Capsule by HPLC-ELSD[J].China Pharmaceuticals,2013,22(14):53-55. |
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| Authors: | Wang Jianfei |
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| Institution: | Wang Jianfei( Wuhu Municipal Institute for Food and Drug Control,Wuhu,Anhui,China 241001) |
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| Abstract: | Objective To establish a method for determining the peiminine content in Chuanbeimo Capsule.Methods The Intersil C 18 column（ 250 mm × 4.6 mm,5 μm） was used with the evaporative light scattering detector（ ELSD） for detection,acetonitrile-0.1% diethylamine as the mobile phase and the fiow rate of 1.0 mL / min.Results The linear range of peiminine was 0.6-6.0 μg（ r = 0.999 5）, and the average recovery rate was 100.16%,RSD = 1.69%.Conclusion The established method is simple,rapid,reliable and accurate for the determination of the peiminine content in Chuanbeimo Capsule,which can be used to control the quality of this praparation. |
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| Keywords: | HPLC-ELSD Chuanbeimo Capsule peiminine |
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