黄芪中黄芪多糖含量测定方法的比较 |
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引用本文: | 杨莉,王志华,陶健生.黄芪中黄芪多糖含量测定方法的比较[J].中国医药工业杂志,2005,36(9):562-563. |
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作者姓名: | 杨莉 王志华 陶健生 |
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作者单位: | 1. 上海交通大学附属瑞金医院药剂科,上海,200025 2. 上海中医药大学药学院,上海,201203 |
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摘 要: | 比较了采用苯酚-浓硫酸比色法和蒽酮-浓硫酸比色法,以葡萄糖和葡聚糖(分子量约20000)两种对照品测定黄芪中多糖含量的方法.结果显示,以葡聚糖为对照品、蒽酮-浓硫酸比色法测定线性关系较好(r=0.9990),平均回收率97.9%,RSD 1.53%.并测定了7个品种黄芪药材中黄芪多糖的含量.
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关 键 词: | 黄芪多糖 苯酚-浓硫酸比色法 蒽酮-浓硫酸比色法 测定 |
文章编号: | 1001-8255(2005)09-0562-02 |
收稿时间: | 2005-01-12 |
修稿时间: | 2005-01-12 |
Comparison of the Methods for Determination of Astraglus Polysaccharides in Radix Astragali |
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Authors: | YANG Li WANG Zhi-Hua TAO Jian-sheng |
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Abstract: | Phenol-sulfuric acid and anthrone-sulfuric acid colorimetric methods for determination of astraglus polysaccharides in Radix Astragali were compared with glucose and dextran(Mw 20000) as the reference, respectively. The results showed that the correlation coefficient of anthrone-sulfuric acid method with the dextran reference was better. The average recovery was 97.9% with RSD of 1.53%. And contents of astraglus polysaccharides in seven different specimens of Radix Astragali were determined by this method. |
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Keywords: | astraglus polysaccharide phenol-sulfuric acid colorimetric method anthrone-sulfuric acid colorimetric method determination |
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