兔血浆α_2－巨球蛋白的分离纯化

本研究从家兔血浆中分离纯化了α＿２－巨球蛋白。首先用利凡诺法从血浆中提取出α＿２巨球蛋白粗制品，然后上ＳｅｐｈａｃｒｙｌＳ＿（４００）凝胶柱过滤后，用琼脂糖凝胶电泳即可得到电泳纯的。α＿２巨球蛋白。结合胰蛋白酶活力测定结果表明，α＿２－巨球蛋白活性峰与ＳｅｐｈａｃｒｙｌＳ＿（４００）凝胶过滤的第一洗脱峰完全重合；ＳＤＳ－聚丙烯酰胺凝胶电泳结果显示出一条单一条带；家兔α＿２－巨球蛋白的亚基分子量经测定为１７８．９４ＫＤ，与人的α＿２－巨球蛋白亚基分子量１８０ＫＤ基本相同。说明用这种方法提取纯化α＿２－巨球蛋白是一种切实可行的方法。

STUDY ON THE ISOLATION AND PURIFICATION OF α_2-MACROGLOBULIN BY RABBIT PLASMA

α_2Macroglobulin was successfully isolated and purified by rabbit plasma.α2-Macroglobulin semifinished product was isolated and extracted from rabbit plasma by therivanol method;the semifinished product was first filtrated by Sephacryl S_400 gel,and then thesample was passed through the electrophoretic purified α_2-macroglobulin was obtained. Theactivity determination combined with trypsin showed that the activity peak of α_2-macroglobu-lin and the first peak of the filtration by Sephacryl S_400 were completely coincided. The tesultin the electrophoresis of Sephacryl S_400 gel showed a single zone. The determination to themolecular weight of α_2-macroglobulin suhunit indicated that the molecular weight ofα_2-macroglobulin subunit in rabbit (178.94 KD) is similar to that in human being (180 KD).