HBsAg转基因小鼠模型的建立及其用于基因治疗研究初探

目的构建HBsAg转基因小鼠模型,并利用其进行基因治疗研究。方法显微注射外源基因pcDNAHBsAg至FVB小鼠原核,注射胚胎移植到同期发情的假孕受体出生个体,经PCR和Southern检测获得阳性转基因小鼠。通过ELISA方法比较分析转基因小鼠经pcDNAHBsAg质粒基因免疫后抗体产生的情况。结果PCR结合Southern检测到了HBsAg阳性小鼠。基因免疫有抗体产生。结论得到的HBsAg转基因小鼠,基因免疫可诱发转基因小鼠产生抗体。

Establishment of HBsAg Transgenic Mice as a Model of Gene Therapy

Objective To study gene therapy through the production of HBsAg transgenic mice model. Methods The pronuclei of FVB mice with exogenous gene, pcDNA HBsAg, were microinjecred, and the injected embryos were transplanted into the recipients. Transgenic positive mice were confirmed by PCR and Southern Blot. ELISA titer was analyzed in transgenic positive mice after gene immunization with pcDNA HBsAg. Results HBsAg transgenic mice were identified by PCR and Southern Blot. The specific antibodies were stimulated after gene immunization. Conclusion HBsAg transgenic mice were produced successfully and gene immunization can stimulate specific antibodies in transgenic mice.