| 畜舍环境中魏氏梭菌分离及基因型鉴定 |
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| 引用本文： | 王海荣 唐德宏 鹿道新 樊丽 柴同杰. 畜舍环境中魏氏梭菌分离及基因型鉴定[J]. 中国人兽共患病杂志, 2005, 21(11): 985-987 |
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| 作者姓名： | 王海荣 唐德宏 鹿道新 樊丽 柴同杰 |
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| 作者单位： | 山东农业大学动物科技学院,山东农业大学动物科技学院,山东农业大学动物科技学院,山东农业大学动物科技学院,山东农业大学动物科技学院 泰安271018,泰安271018,泰安271018,泰安271018,泰安271018 |
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| 摘 要： | 目的从山东省泰安、青岛等地6个市的2个牛舍、4个猪舍、5个兔舍空气及畜舍动物粪便中分离魏氏梭菌,从空气中分离到66株,从畜舍粪便中分离到70株,通过多重聚合酶链反应(multi-PCR)对分离到的魏氏梭菌进行型别的鉴定,结果发现:分离到的魏氏梭菌均为A型。
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| 关 键 词： | 魏氏梭菌 多重聚合酶链反应 A型 畜舍环境 |
| 文章编号： | 1002-2694(2005)11-0985-03 |
| 收稿时间： | 2005-03-10 |
| 修稿时间： | 2005-03-10 |
Isolation and genotypic identification of Clostridium perfringens from environments of animal houses |
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| WANG Hai-rong, TANG De-hong, LU Dao-xin, FAN Li, CHAI Tong-jie. Isolation and genotypic identification of Clostridium perfringens from environments of animal houses[J]. Chinese Journal of Zoonoses, 2005, 21(11): 985-987 |
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| Authors: | WANG Hai-rong TANG De-hong LU Dao-xin FAN Li CHAI Tong-jie |
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| Affiliation: | Department of Animal Husbandry and Veterinary Medicine, Shandong Agricultural University, Tai an, 271018, China |
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| Abstract: | Sixty six strains and 70 strains of Clostridium perfringens were isolated by culture method from 500 air samples and 380 fecal specimens of animals respectively, and these strains of bacteria were subjected to identification by means of multiplex PCR technique. For detecting the types of C. perfringens, 4 pairs of primers for alpha, beta, epsilon and jota toxin genes were designed according to the published data on the corresponding sequences., and the multiplex PCR was used for the identification of the toxin types of C. perfringens isolated from animal houses in Shandong province. The results showed that the strains of C. perfringens isolated all belonged to A type, that produced alpha toxin. |
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| Keywords: | Clostridium perfringens toxin type A Multiplex PCR environment of animal house |
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