银杏花粉的部分纯化和变应原性、免疫原性分析

作者采用层析法对银杏花粉进行部分纯化。将各组分对40例变应性鼻炎或/和哮喘患者进行皮试,结合ELISA抑制试验测试其对特异性IgE和IgG的抑制率,阐明各组分具有不同的变应原活性和免疫原活性。为临床应用提出了初步意见。

Partial Purification and Analysis of Allergenicity,Immunogeniciy of Ginkgo biloba L. Pollen

Pollens of Ginkgo biloba L.(G. b.l.p) have been found to be a kind ofimportant allergen which causes pollinosisin Chengdu.The goal of thisstudy is to purifyG. b.l.p and to determine the allergenicityand immunogenicity of various fractions.Crude extract was purified by gel filtrationwith Sephadex G25, then G75.Two elutionpeaks were beserved. On SDS--PAGE, themolecular weights of protein of the 1st peakand the valley were 30--42 kd and 13--18kd,respectively, and that of the 2nd peat wasless than 13 kd. 40 patients with allergic rhinitis and/orasthma underwent the skin test with crudeextract and various fractions of gel filtra-tion it revealed that the strongest allergenicactivity existed in the 1st peak and therewas mild allergenic activity in the 2ndpeak. The in vitro allergenic activity andimmunogenic activity of various fractionswere examined by ELISA inhibition test.It was further confirmed that the allergenicactivity and immunogenic activity of the1st peak were the strongest, and those ofthe 2nd peak were the lowest. It is suggested that diagnosing reagentscan be made satisfactorily by partial puri-fication,i.e. discarding the inactive frac-tions, since allergenicity exists in variousfragments. But fractions of allergen withhigh IgG immunogenicity should be selectedto produce immunotherapy agents so as toenhance the production of blocking antibodyand thus improve the therapeutic effect.