复方苯妥英钠凝胶剂对创面修复和细菌感染的影响

目的:观察复方苯妥英钠凝胶剂对实验性创面修复及细菌感染的影响.方法:建立小鼠皮肤切割伤模型,用复方苯妥英钠凝胶剂低、高剂量(4.25,4.55 g·kg-1)于创面涂药,连续12d,分别于3,6,8,10,12 d观察小鼠皮肤创面的收缩率;建立大鼠皮肤切割伴金黄色葡萄球菌感染模型,用复方苯妥英钠凝胶剂低、高剂量(1.70,1.82 g·kg-1)于创面涂药,连续12 d,观察大鼠皮肤创面的愈合率,同时对愈合组织进行病理学组织学检查.结果:3,6,8,10,12 d,复方苯妥英钠凝胶剂低、高剂量组小鼠创面收缩率分别为(20.16 ±5.78)％,(40.02 ±9.79)％,(71.04±7.27)％,(96.14±2.53)％,(99.44±1.30)％,(28.50±4.77)％,(55.66±8.39)％,(79.67±5.11)％,(96.14±2.53)％,(100.00±0.00)％,明显高于凝胶基质组(16.77±4.79)％,(29.95±5.63)％,(50.69±4.19)％,(67.47±5.13)％,(82.48±4.40)％(P＜0.05,P＜0.01,P＜0.01,P＜0.01;P＜0.01,P＜0.01,P＜0.01,P＜0.01,P＜0.01);复方苯妥英钠凝胶剂低、高剂量组大鼠皮肤创面的愈合率分别为(29.45±5.11)％,(50.17±4.29)％,(72.48±5.05)％,(87.44±4.11)％,(96.66±3.75)％;(33.71±3.43)％,(58.67±5.59)％,(81.86 ±4.15)％,(95.92±2.39)％,(100.00±0.00)％明显高于凝胶基质组(20.71±3.58)％,(40.42±3.89)％,(58.53±6.21)％,(74.71±9.23)％,(86.47±7.15)％(均P＜0.01).结论:复方苯妥英钠凝胶剂能促进实验性细菌感染创面的愈合.

Effect of Compound Phenytoin Sodium Gel on Restoration of the Wounds Surface and Bacterial Infection

Objective: To observe the influence of compound phenytoin sodium gel (CPSG) for experimental animals on restoration of the wound's surface and bacterial infection. Method: The incised wound model of mice was established, CPSG (4.25,4.55 g·kg^-1)was external applied for 12 days and the wound contraction rate were observed after treatment for 3, 6,8,10, 12 days. Rats incised wound model accompanied by staphylococcus aureus were replicated, CPSG (1.70,1.82 g·kg^-1)was external applied for 12 d, and the wound recovery rate were detected after treatment for 3, 6,8,10,12 days. The histopathological changes of wound healing tissue were examined. Result: Compared with the wound contraction rate of gel group (16.77±4.79)%, (29.95±5.63)%,(50.69±4.19)%,(67.47±5.13)%,(82.48±4.40)%, those in low and high doses of CPSG were (20.16±5.78)%,(40.02±9.79)%,(71.04±7.27)%,(96.14±2.53)%,(99.44±1.30)%;(28.50±4.77)%,(55.66±8.39)%,(79.67±5.11)%,(96.14±2.53)%,(100.00±0.00)% respectively(P<0.05,P<0.01,P<0.01,P<0.01. P<0.01,P<0.01,P<0.01,P<0.01,P<0.01)after treatment for 3, 6,8,10,12 days in mice. The wound recovery rate of the low and high dose of CPSG were (29.45±5.11)%,(50.17±4.92)%,(72.48±5.05)%, (87.44±4.11)%,(96.66±3.75)%;(33.71±3.43)%,(58.67±5.59)%,(81.86±4.15)%,(95.92±2.39)%,(100.00±0.00)% higher than (20.71±3.58)%,(40.42±3.89)%,(58.53±6.21)%,(74.71±9.23)%,(86.47±7.15)% of gel group(all P<0.01)in the treatment of 3, 6, 8,10, 12 days. Conclusion: CPSG could promote the restoration of the wound's surface in the animals with experimental trauma and bacterial infection.