枳实消痞丸诱导胃癌SGC-7901细胞凋亡的研究

目的:从细胞增殖、凋亡和周期角度研究枳实消痞丸治疗胃癌的机制.方法:胃癌SGC-7901细胞阴性对照组加完全培养基,1 ×104细胞/孔接种于96孔板,将枳实消痞丸分为4,2,0.5,0.125,0.05 g·L-15个质量浓度加入,分别作用24,48,72 h,MTT法检测细胞增殖;1×105/孔接种于6孔板,加入2,0.5,0.125 g·L-13个质量浓度的药物,分别培养48,72 h,流式细胞仪检测细胞周期和细胞凋亡.结果:枳实消痞丸4,2,0.5,0.125,0.05 g·L-1质量浓度作用于胃癌SGC-7901细胞,抑制率依次降低;作用于24,48,72 h,抑制率渐增.枳实消痞丸增加了G0/G1期的细胞比率,促进细胞凋亡,高、中浓度强于低浓度.结论:枳实消痞丸抑制了细胞的增殖,具有时间和浓度依赖性,其机制可能与药物阻滞细胞于G0/G1期和诱导细胞凋亡有关.

Studying of Apoptosis of SGC-7901 Cell Induced by Zhishi Xiaopi Wan

Objective: Study on the mechanism of Zhishi Xiaopi Wan treatment on Gastric cancer from cell proliferation, apoptosis and cycle. Method: Complete medium was mixed in negative control group;1×10⁴ cells/wells were seeded at 96 wells plate and divided into negative control, Zhishi Xiaopi Wan in 0.05,0.125,0.5,2,4 g·L^-1 concentration groups;the cells were treated with drugs for 24,48,72 h, and cell proliferation was detected by MTT method; 1×10⁵ cells/wells were seeded in 6 wells plate, and the drugs of low(0.125 g·L^-1),medium(0.5 g·L^-1),high(2 g·L^-1) concentration respectively were put into wells for 48,72 h, then the cell cycle and apoptosis of gastric cancer SGC-7901 cells were detected by flow cytometry(FCM). Result: Gastric cancer SGC-7901 cells were treated by the 0.125,0.5,2 g·L^-1 of Zhishi Xiaopi Wan, and its inhibition rate is in dose and time dependent. Zhishi Xiaopi Wan could increase SGC-7901 cells in G₀-G₁ phase percentage and promote apoptosis; The high,medium concentration was stronger than the lows. Conclusion: Zhishi Xiaopi Wan can inhibit the proliferation of SGC-7901 cell and there is in dose and time dependent, which mechanism may be related to drug block of cells to G₀/G₁ phase and induce cell apoptosis.