异型南五味子醇提物对四氯化碳致大鼠急性肝损伤的保护作用

目的:探讨异型南五味子(血筒)醇提物对四氯化碳(CCl4)所致大鼠急性肝损伤的保护作用.方法:SD大鼠随机分为正常组,模型组,联苯双酯组,血筒醇提物高、中、低剂量组,用药组连续ig给药7d.第6天除正常组皮下注射等量生理盐水外,其他各组均皮下注射CCl4.24 h后,检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性,取肝脏计算肝指数,制备肝匀浆测定还原型谷胱甘肽(GSH)含量,于光镜下观察肝组织的病理变化.结果:血筒醇提物低、中、高剂量组能不同程度地降低急性肝损伤大鼠肝脏指数及ALT,AST水平,升高肝组织GSH水平(P＜0.05或P＜0.01),减轻CCl4引起的肝组织损伤程度.结论:血筒醇提物对CCl4致大鼠急性肝损伤有明显的保护作用.

Protective Effects of Alcohol Extract of Kadsura heteroclita on Acute Hepatic Injury Induced by Carbon Tetrachloride in Rats

Objective: To observe the protective effects of alcohol extract of Kadsura heteroclita (Roxb) Craib on acute hepatic injury induced by carbon tetrachloride in rats. Method: SD rats were randomly divided into the normal group, model group, bifendate group, and alcohol extract of K. heteroclita low, middle, high dosage groups. In sixth day, other rats were injected subcutaneously with CCl₄ except the normal group with the same volume of saline after they were performed by gavage once a day and lasted for 7 days. After injected 24 hours, the contents of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum, the liver index and contents of reduced glutathione(GSH) in liver tissue were measured and the liver pathological changs were observed. Result: The alcohol extract of K. heteroclita decreased significantly the levels of serum ALT and AST and liver index, and increased the level of GSH (P<0.05 or P<0.01). The pathological changes were relieved in the treatment groups. Conclusion: The alcohol extract of K. heteroclita has protective effects on acute hepatic injury induced by carbon tetrachloride in rats.