颈动脉粥样硬化超声表现与同型半胱氨酸及MTHFR基因多态性的相关性

目的 探讨颈动脉粥样硬化超声表现与血浆同型半胱氨酸(Hcy)及MTHFR C677T基因多态性之间的相关性。方法 143例颈动脉粥样硬化患者作为动脉硬化组,再进一步细分为内膜增厚亚组(75例)与斑块亚组(68例),选择91名无颈动脉内膜增厚及斑块形成者作为对照组。对所有研究对象均进行血浆Hcy及MTHFR C677T基因多态性检测。结果 MTHFR C677T基因CC、CT及TT型血浆Hcy水平依次逐渐升高,各组间差异有统计学意义(P<0.05)。血浆Hcy是颈动脉粥样硬化的独立危险因素(P<0.05),而MTHFR C677T基因多态性未进入回归方程。结论 血浆Hcy升高是颈动脉粥样硬化的独立危险因素;MTHFR C677T基因多态性在颈动脉粥样硬化与正常人群中分布不同,并与血浆Hcy水平相关,但不是颈动脉粥样硬化的独立危险因素。

Relationship of ultrasonic manifestations with homocysteine and polymorphism of MTHFR gene in patients with carotid atherosclerosis

Objective To evaluate the relationship of ultrasonic manifestations, plasma homocysteine (Hcy) and polymorphism of MTHFR C677T gene in patients of carotid atherosclerosis (CAS). Methods Totally 143 CAS patients were included as CAS group, and were further divided into increased intima-media thickness (IMT) subgroup (75 patients) and carotid plaque subgroup (68 patients). Ninety-one subjects had no increased IMT nor carotid plaque were enrolled in the control group. Plasma Hcy level were measured and MTHFR C677T genotype were detected. Results Plasma Hcy level in groups with CC, CT or TT genotype increased gradually (P<0.05). Hcy was an independent factor to CAS (P<0.05). Polymorphism of MTHFR C677T gene did not enter into the regression equation. Conclusion Plasma Hcy level increasing is an independent factor to carotid atherosclerosis. Polymorphism of MTHFR C677T gene is different in carotid atherosclerosis patients and normal population, which is associated with plasma Hcy level, but is not an independent factor to carotid atherosclerosis.