A variation of the tissue print technique for studying isolated spinal cord cells in situ |
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Authors: | SC MacDonald S Hochman |
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Institution: | Department of Physiology, 420 BMSB, University of Manitoba, 770 Bannatyne Avenue, Winnipeg, Manitoba, R3E 0W3, Canada |
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Abstract: | We present a novel variation of the tissue print technique which yields isolated spinal cells that retain their original topographical organization in situ. A light papain digestion combined with a low-melting-point agarose as the adhesive substrate is used to ‘print' a monolayer of cells from spinal cord slices. Printed cells are viable for studies using electrophysiological approaches. This technique provides a means to bridge the gap between properties of isolated cells and their topographical identity in spinal cord. |
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Keywords: | spinal cord |
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