益气化瘀中药对糖尿病皮肤溃疡大鼠转化生长因子β1的影响

目的:探讨益气化瘀中药及其拆方(益气方和化瘀方)促进糖尿病溃疡创面愈合的分子机制。方法:将60只大鼠随机分为益气化瘀组、益气组、化瘀组、贝复济组、模型组和正常对照组。通过腹腔注射链脲佐菌素和外科方法建立糖尿病大鼠全层皮肤缺损模型,给予不同中药煎剂口服及贝复济外用对照,运用免疫组织化学、图像分析和Western blotting的技术手段分别检测各组治疗后创面新生肉芽组织中转化生长因子β1(transforming growth factor-β1,TGF-β1)的表达水平。结果:糖尿病溃疡模型组TGF-β1表达水平低于正常对照组(P<0.01);各治疗组TGF-β1表达水平高于糖尿病溃疡模型组(P<0.01);益气化瘀组TGF-β1表达水平高于拆方组和贝复济组(P<0.05)。结论:TGF-β1表达水平下降可能是糖尿病创面难以愈合的机制之一,益气化瘀中药可能是通过提高创面TGF-β1的表达水平,促进创面愈合。

Effects of Chinese herbs for replenishing qi and resolving stagnation on transforming growth factor-β1 of skin ulcers in rats with diabetes

OBJECTIVE: To explore the effects of Yiqi Huayu Recipe, a compound traditional Chinese herbal medicine for replenishing qi and resolving stagnation, on transforming growth factor-beta1(TGF-beta1) of skin ulcers in rats with diabetes. METHODS: Sixty rats were randomly divided into six groups, Yiqi Huayu Recipe-treated group, Yiqi (replenishing qi) Recipe-treated group, Huayu (resolving stagnation) Recipe-treated group, basic fibroblast growth factor (bFGF) treated group, untreated group and normal control group. Diabetes was induced by peritoneal injection of streptozotocin and skin ulcers were made by surgery method in rats except for the normal control group. Then the rats were administered with different drugs respectively, and the expression of TGF-beta1 in granulation tissue of the skin ulcers was detected with the methods of Western blotting, image analysis and immunohistochemistry. RESULTS: The level of TGF-beta1 expression in the untreated group was lower than that in the normal control group (P<0.01); and the level of TGF-beta1 expression in the drug-treated groups was higher than that in the untreated group (P<0.01); and the TGF-beta1 expression in the Yiqi Huayu Recipe-treated group was higher than that in the Yiqi Recipe-treated group, Huayu Recipe-treated group and bFGF-treated group (P<0.05). CONCLUSION: The replenishing qi and resolving stagnation therapy can control the secretion of TGF-beta1 of the wound in the process of wound healing in the levels of gene and molecule.