恒河猴IL-4与变异IL-4促进外周血单核细胞向树突状细胞转化功能的比较

 【目的】比较重组的恒河猴IL-4与变异IL-4在促进外周血单核细胞向树突状细胞转化方面功能的异同。【方法】 利用葡聚糖-泛影葡胺梯度离心法分离恒河猴外周血单个核细胞,阴性筛选非CD3+细胞,随后贴壁分离外周血单核细胞并进行流式细胞技术确定筛选结果。将所分离的外周血单核细胞分为阴性对照组,IL-4+GM-CSF组和vIL-4+GM-CSF组进行体外培养,收集细胞并表面染色CD14、CD1a、CD11c和CD83进行流式细胞分析。【结果】 利用三步分离的方法得到了纯度达90%的未激活的外周血单核细胞。培养后的细胞进行流式分析,结果提示与阴性对照组相比,IL-4+GM-CSF组和vIL-4+GM-CSF组细胞的CD14表达明显下降,而CD1a、CD11c和CD83表达明显上调,两实验组间无差别。【结论】 与IL-4相同,vIL-4亦能促进外周血单核细胞转化为树突状细胞。

Compare Effect of Macaca Mulatta IL-4 and Variant IL-4 on Transforming Peripheral Blood Monocytes to Dendritic Cells

[Objective] To compare effect of Macaca mulatta IL-4 and variant IL-4(vIL-4) on transforming peripheral blood monocytes to dendritic cells.[Methods] Macaca mulatta PBMCs were isolated by Ficoll/Hypaque density gradient centrifugation,then magnetic activated cell sorter(MACS) CD3 MicroBead Kit(non-human primate) was used to negatively selected non-CD3+ cells,after that,peripheral blood monocytes were separated by attaching to plate wall and analyzed by flow cytometry to identify.Monocyte enriched cells were ...