DEVELOPMENT OF AN EFFICIENT AND SIMPLE METHOD FOR CONJUGATION OF LACCASE TO IMMUNOGLOBULIN AND ITS CHARACTERIZATION BY ENZYME IMMUNOASSAY |
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| Authors: | Swarnadeep Ray Protik Chowdhury Nirmalendu Das |
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| Affiliation: | 1. Department of Physiology , Immunology Laboratory, University of Calcutta , Calcutta, India;2. Department of Botany , ABN Seal College , Cooch Behar, West Bengal, India |
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| Abstract: | A new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi Pleurotus ostreatus. The efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. The assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody. |
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| Keywords: | EIA ELISA enzyme antibody conjugate immunoglobulin laccase Pleurotus |
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