uPA、PAI-1在食管癌发生、发展不同阶段的表达及意义

目的:探讨尿激酶型纤溶酶原激活物(uPA)、纤溶酶原激活物抑制剂-1(PAI-1)在食管癌发生、发展中的作用及意义。方法:采用免疫组织化学SP法检测正常食管黏膜上皮组织(18例)、食管黏膜上皮不典型增生组织(23例)及食管癌组织(68例)中uPA、PAI-1蛋白的表达,并分析其和食管癌临床病理特征的关系。结果:uPA蛋白在正常食管黏膜上皮组织、食管黏膜上皮不典型增生组织及食管癌组织中的阳性率分别为27.8%、39.1%、70.6%,三者间有显著性差异(P<0.05);PAI-1蛋白在正常食管黏膜上皮组织、食管黏膜上皮不典型增生组织及食管癌组织中的阳性率分别为22.2%、34.8%、64.7%,三者间有显著性差异(P<0.05)。uPA、PAI-1蛋白表达与年龄、性别、病理类型无关(P>0.05),在临床病理Ⅰ-Ⅱ期和Ⅲ-Ⅳ期间有升高的趋势,但差异无统计学意义(P>0.05),与分化程度和淋巴结有无转移有关(P<0.05)。结论:uPA、PAI-1蛋白在食管癌发生、发展不同阶段的表达呈进行性上升的趋势,可能与食管癌的发生、发展相关,过度表达提示预后不良。

Expression of uPA and PAI-1 in different stages of carcinogenesis and progression of esophageal cancer

Objective:To investigate the effects of uPA and PAI-1 in carcinogenesis and progression of esophageal carcinoma.Methods:The expression of uPA and PAI-1 in the tissue of normal(18 cases),atypical hyperplasia(23 cases) and esophageal carcinoma(68 cases) were evaluated by SP immunohistochemistry.uPA and PAI-1 expression was assessed as to pathologically biological features of esophageal cancer.Results:The positive rates of uPA were 27.8%,39.1%,70.6% in the tissue of normal,atypical hyperplasia and esophageal carcinoma respectively,there were significant difference in these three expressions(P<0.005).The positive rates of PAI-1 were 22.2%,34.8%,64.7% in the tissue of normal,atypical hyperplasia and esophageal carcinoma respectively,there were significant difference in these three expressions(P<0.005).These two expressions,uPA and PAI-1,in malignant tumors weren’t associated with age,gender and pathological types,but associated with histologic grading and lymph node metastasis.The positive rates of uPA and PAI-1 were higher in the clinical stage Ⅲ-Ⅳ than that in the clinical stageⅠ-Ⅱ,though the difference is not significant(P>0.05).Conclusion:Rising expressions of uPA and PAI-1 are common in tumorigenesis and progression of esophageal carcinoma.Altered expression of uPA and PAI-1 may contribute to tumorigenesis and progression of esophageal carcinoma,its overexpression indicate worse prognosis.