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| 脂多糖刺激前后树突状细胞CD44、CD24基因的表达 | |
| 引用本文： | 郑瑾,刘强,李燕,杨建栋,马骥,刘尧,刘文超.脂多糖刺激前后树突状细胞CD44、CD24基因的表达[J].现代肿瘤医学,2011,19(3):407-410. |
| 作者姓名： | 郑瑾 刘强 李燕 杨建栋 马骥 刘尧 刘文超 |
| 作者单位： | 1. 第四军医大学西京医院肿瘤中心,陕西,西安,710032 2. 第四军医大学唐都医院血液科,陕西,西安,710038 3. 第四军医大学生物化学及分子生物学教研室,陕西,西安,710032 4. 第四军医大学西京医院肝胆外科,陕西,西安,710032 |
| 摘 要： | 目的:检测脂多糖作用于树突状细胞前后CD44、CD24基因的表达情况。方法:从外周血分离单个核细胞,加入rhGM-CSF及rhIL-4,培养的第6天,实验组加入脂多糖刺激24 h而对照组不加,于刺激后不同时间点分别提取细胞总RNA,行real time PCR检测。结果:单个核细胞经GM-CSF及IL-4诱导6d后分化成为树突状细胞,表达较低水平的CD44及CD24。脂多糖刺激后树突状细胞的CD44及CD24表达均较对照组增高,CD44增高更明显。结论:树突状细胞受脂多糖刺激后CD44及CD24表达显著增高,这有利于DC-T细胞簇的形成及抗原的递呈,从而导致DC免疫功能的增强。 |
| 关 键 词： | 脂多糖 树突状细胞 CD44 CD24 实时定量PCR |
Detection of genes expression for CD44 and CD24 on dendritic cells before and after stimulation by lipopolysaccharide |
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| ZHENG Jin,LIU Qiang,LI Yan,YANG Jian-dong,MA Ji,LIU Yao,LIU Wen-chao.Detection of genes expression for CD44 and CD24 on dendritic cells before and after stimulation by lipopolysaccharide[J].Journal of Modern Oncology,2011,19(3):407-410. | |
| Authors: | ZHENG Jin LIU Qiang LI Yan YANG Jian-dong MA Ji LIU Yao LIU Wen-chao |
| Institution: | 1Department of Oncology,Xijing Hospital,the Fourth Military Medical University,Xi’an 710032,China;2 Department of Hematology,Tangdu Hospital,the Fourth Military Medical University,Xi’an 710038,China;3Department of Biochemistry and Molecular Biology,the Fourth Military Medical University,Xi’an 710032,China;4 Department of Hepatobiliary Surgery,Xijing Hospital,the Fourth Military Medical University,Xi’an 710032,China. |
| Abstract: | Objective:To detect the genes expression for CD44 and CD24 on dendritic cells(DC) before and after stimulation by lipopolysaccharide(LPS).Methods:Human monocytes were isolated from heparinized whole blood and then induced into DC by adding rhGM-CSF and rhIL-4 for 6d.After that,DCs were induced with or without LPS for 24h and total cellular RNA were extracted for real time PCR analysis.Results:Expressions of CD44 and CD24 were lower in monocytes-derived DC induced by rhGM-CSF and rhIL-4 for 6d.After being stimulated by LPS for 24h,the expression of CD44 and CD24 increased significantly(P<0.05).Conclusion:The expression of CD44 and CD24 on DC increased after being stimulated by LPS.This is related to functional maturation of DC. |
| Keywords: | CD44 CD24 |
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