肿瘤细胞在活体小鼠肝脏微循环中动态变化的可视性研究

目的:在小鼠活体内动态观察肿瘤细胞在肝脏微循环中的运动、分布及凋亡。方法:用Calcein AM荧光标记B16F1黑色素瘤细胞,将其注射入C57BL/6小鼠的肠系膜静脉,在荧光倒置显微镜下动态观察肿瘤细胞在肝脏微循环中的运动及分布,测量并计算肿瘤细胞在终末门静脉中的运动速度。注射肿瘤细胞8小时后,切取肝脏,用凋亡标记荧光素对肝组织切片进行原位DNA末端标记,在荧光显微镜下计算凋亡细胞百分比。结果:B16F1黑色素瘤细胞进入肝脏微循环中后,90%的细胞通过机械性嵌顿停留在肝窦中,10%的肿瘤细胞通过黏附停留在终末门静脉中,B16F1细胞在肝脏终末门静脉中的运动速度为648±53μm/s。在注射肿瘤细胞8小时后,在肝窦中约有30%的肿瘤细胞发生凋亡,在终末门静脉中约有10%的肿瘤细胞发生凋亡。结论:利用荧光标记的肿瘤细胞及荧光倒置显微镜为探讨肿瘤肝转移机制提供了一个良好的活体观察方法。

Visualization of interactions between tumor cells and hepatic microvasculature of mice in vivo

Objective:To detect the movement,dispersion and apoptosis of tumor cells in the hepatic microvasculature of mice in vivo.Methods:Calcein AM labeled B16F1 melanoma cells were injected into mesenteric vein of the C57BL / 6 mice.The movement and distribution of tumor cells in the hepatic microvasculature of mice were detected by fluorescence inverted microscope.The velocity of tumor cells moving in the terminal portal vein was measured.And apoptosis of tumor cells in hepatic microvasculature were calculated 8 ...