| 5-氮杂脱氧胞苷酸诱导ER阴性细胞ERα,ERβ恢复表达的实验研究 |
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| 引用本文： | 赵琳,于兆进,李宇男,何苗,王麟,任婕,白雪峰,赵海山,姚维范,魏敏杰.5-氮杂脱氧胞苷酸诱导ER阴性细胞ERα,ERβ恢复表达的实验研究[J].现代肿瘤医学,2011,19(5):842-845. |
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| 作者姓名： | 赵琳 于兆进 李宇男 何苗 王麟 任婕 白雪峰 赵海山 姚维范 魏敏杰 |
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| 作者单位： | 中国医科大学药学院药理教研室,辽宁,沈阳,110001 |
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| 基金项目： | 国家自然科学基金资助项目，国家重大新药创制平台资助项目，辽宁省科技厅科学技术计划资助项目，辽宁省博士科研启动基金资助项目 |
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| 摘 要： |
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| 关 键 词： | 5-氮杂脱氧胞苷酸 乳腺癌 雌激素受体 甲基化 |
Investigation of 5-Aza-2'-deoxycytidine inducing ERα, ERβ expresssion |
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| ZHAO Lin,YU Zhao-jin,LI Yu-nan,HE Miao,WANG Lin,REN Jie,BAI Xue-feng,ZHAO Hai-shan,YAO Wei-fan,WEI Min-jie.Investigation of 5-Aza-2'-deoxycytidine inducing ERα, ERβ expresssion[J].Journal of Modern Oncology,2011,19(5):842-845. |
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| Authors: | ZHAO Lin YU Zhao-jin LI Yu-nan HE Miao WANG Lin REN Jie BAI Xue-feng ZHAO Hai-shan YAO Wei-fan WEI Min-jie |
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| Institution: | ZHAO Lin,YU Zhao-jin,LI Yu-nan,HE Miao,WANG Lin,REN Jie,BAI Xue-feng,ZHAO Hai-shan,YAO Wei-fan,WEI Min-jie Department of Pharmacology,College of Pharmacy,China Medical University,Shenyang 110001,China. |
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| Abstract: | Objective:To investigate the effect of 5-Aza-2’-deoxycytidine inducing ERα,ERβ re-expression in ERα,ERβ negative MDA-MB-435 breast cancer cell line.Methods:The level of DNMT1,ERα and ERβ mRNA in ERα,ERβ negative MDA-MB-435 breast cancer cell line before or after treated with 5-Aza-dC were tested by RT-PCR.Results: Compared with vehical group,cells treated with different concentration of 5-Aza-dC(1,2.5,5,10,20μmol/L) DNMT1 mRNA expression decreased.After been exposed to 2.5,5,10,20μmol/L 5-Aza-dC respectively ERα,ERβ mRNA expression were resorted in a dose dependent manner,there was statistically diffrence(P<0.05).Conclusion: The expression of ERα,ERβ mRNA in ERα,ERβ negative breast cancer cell line coud be resorted by 5-Aza-dC in a dose dependent manner.So,aberrant methylation was one of the main mechanisms of inducing the loss of ERα,ERβ expression. |
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| Keywords: | 5-Aza-2'-deoxycytidine breast cancer estrogen receptor methylation |
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