子宫内膜异位症腹腔液对精子运动功能影响的体外研究

目的　研究子宫内膜异位症 (内异症 )患者腹腔液体外对精子运动功能的影响 ,以探讨内异症引起不孕的机制。方法　取正常精液标本 ,经上游优化处理后 ,与内异症组 (30例 )及对照组 (18例 )腹腔液孵育 2h、4h ,采用计算机辅助精子分析系统 (CASA)检测精子的运动参数。结果　精子与腹腔液孵育 4h后 ,内异症组的精子总活动率、前向运动精子百分率、快速前向运动精子百分率及曲线速度 (VCL)、路径速度 (VAP)、直线速度 (VSL)、直线性(LIN)、摆动性 (WOB)、前向性 (STR)均显著低于对照组 (P <0 .0 1)。结论　内异症患者腹腔液对精子活动的抑制可能是导致不孕的一个因素。

Influence of peritoneal fluid in patients with endometriosis on sperm motility in vitro

ObjectiveTo evaluate the influence of peritoneal fluid in patients with endometriosis(EM) on human sperm motility in vitro,and further analyze the pathogenesis of infertility associated with EM.Methods Normal sperm aseptically prepared by swim-up technique was incubated with peritoneal fluid obtained from EM group(30 women) and control group(18 women).Measurements were carried out in a session of 2 h and 4 h in all specimens with computer aided sperm analysis(CASA).Results After 4 h incubation with peritoneal fluids,the sperm motive parameters were decreased significantly in EM group(P<0.01) including total sperm motility,sperm progressive motility,sperm fast progressive motility and VCL,VAP,VSL,LIN,WOB,STR. Conclusion It is likely that reduction of sperm motility in peritoneal fluid in patients with EM may play a role in the pathogenesis of infertility associated with EM.