体外培养兔视网膜Müller细胞葡萄糖转运载体蛋白1表达及其影响因素研究

目的观察高糖以及高浓度胰岛素等不同因素对体外培养兔视网膜Müller细胞葡萄糖转运载体蛋白1(GLUT1)表达的影响。方法利用组织块悬浮法原代培养兔视网膜Müller细胞，分为正常对照组、高糖组、胰岛素组、高糖加胰岛素组，通过激光共聚焦显微镜结合免疫细胞化学、荧光染色的方法，定位定量分析GLUT1的表达情况。结果高糖及高浓度胰岛素条件下视网膜Müller细胞GLUT1的表达均明显增强，并主要位于细胞浆靠近核膜周围。结论视网膜Müller细胞可表达GLUT1，高糖以及高浓度胰岛素均可使其表达增强。 （中华眼底病杂志，2008，24：265-267）

Study of expression and effect factors on glucose transporter-1 of rabbit retinal Muller cells cultured in vitro

Objective To observe the different effect such as high coneentration of glucose and high concentration of insulin on GLUT1 of Rabbit Retinal Mailer Cell in vitro. Methods Rabbit retinal Muller cells were cultured in vitro with suspended constitution, which were divided as the following groups: common control group, high glucose group,insulin group, high glueose combined insulin group. Laser confocal microscope combined with immunocytochemical and fluorescence staining method to quantitatively analyze the expression condition of GLUT1. Results The expression of GLUT1 has been enhanced obviously by high glucose and high insulin,which locates mainly in the cytoplasm that near to the nucleus. Conclusion Rabbit retinal Mailer cells can express GLUTI,and the expression of GLUT1 can be reinforced by high glucose and high insulin.