| Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats |
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| 引用本文： | 国华,梁华平,吕凤林,徐祥,安兵,郝天智,王付龙,杨文军.Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats[J].中华创伤杂志(英文版),2003,6(6):363-369. |
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| 作者姓名： | 国华 梁华平 吕凤林 徐祥 安兵 郝天智 王付龙 杨文军 |
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| 作者单位： | ResearchInstituteofSurgery,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China |
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| 基金项目： | ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina (No .30 170 36 7),theTenth FiveYearPlanofMilitaryCommand (No .0 1MB113)andMajorBasicResearchDevelopmentProgramofChina (No .G19990 5 4 2 0 3) |
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| 摘 要： | Objective: To study the effect of decoyoligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-KB) cis-elements on expression of inflammation mediators in pMcP cells from rats. Methods： With carriers of cationic liposomes, decoy-ODNs were transfected into pMΦ cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and IL-10 were analyzed. Results. Decoy-ODNs could decrease the expression of TNFα and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10. Conclusions: Decoy-ODNs targeting NF-κB can decrease the expression of inflammatory mediators in pMΦ cells from rats.
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| 关 键 词： | 炎症 炎性介质 细胞因子 decoy-ODNs pMΦ细胞 白细胞介素 |
Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats |
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| Hua Guo,Hua-ping Liang,Feng-lin Lu,Xiang Xu,Bin An,Tian-zhi Hao,Fu-lLong Wang,Wen-jun Yang.Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats[J].Chinese Journal of Traumatology(English Edition),2003,6(6):363-369. |
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| Authors: | Hua Guo Hua-ping Liang Feng-lin Lu Xiang Xu Bin An Tian-zhi Hao Fu-lLong Wang Wen-jun Yang |
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| Institution: | Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China |
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| Abstract: | OBJECTIVE: To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats. METHODS: With carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed. RESULTS: Decoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10. CONCLUSIONS: Decoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats. |
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| Keywords: | Tumor necrosis factor Inflammation mediators Nuclear factor kappa B Decoy-oligodeoxynucleotides |
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