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| Effect of nitric oxide synthase inhibitor on proteoglycan metabolism in repaired articular cartilage in rabbits | |
| 引用本文： | 孙炜,金大地,王吉兴,秦立赟,刘晓霞. Effect of nitric oxide synthase inhibitor on proteoglycan metabolism in repaired articular cartilage in rabbits[J]. 中华创伤杂志(英文版), 2003, 6(6): 336-340 |
| 作者姓名： | 孙炜 金大地 王吉兴 秦立赟 刘晓霞 |
| 作者单位： | Department of Traumatic Surgery，Shenzhen Second People's Hospital，Department of Orthopaedics & Spine Surgery，Nanfang Hospital，First Military Medical University，Department of Orthopaedics & Spine Surgery，Nanfang Hospital，First Military Medical University，Department of Surgery，188th Hospital of PLA，Department of Orthopaedics & Spine Surgery，Nanfang Hospital，First Military Medical University Shenzhen 518025，China，Guangzhou 510515，China，Guangzhou 510515，China，Chaozhou 515000，China，Guangzhou 510515，China |
| 基金项目： | ThisprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No .3990 0 14 9) |
| 摘 要： | Nitricoxide (NO )isanimportantregulatoryfactorandasignal transductionmoleculeintheprocessofcellgrowthanddifferentiation .Recentreportshaveindicatedthatnitricoxideiscloselyrelatedtothedisintegrationofarticularcartilage,becausehighexpressionofinducednitricoxidesynthase (iNOS)inhibitormRNAisdetectedinthearticularcartilageandiNOScanamelioratethecartilagemetabolisminpatientswithosteoarthritisorrheumaticarthritis .1,2 OurpreviousstudyshowedthatiNOScanamelioratethe qualityofrepairedcartilages ,t… |
| 关 键 词： | 蛋白多糖 关节软骨 创伤 动物实验 一氧化氮合成酶抑制剂 组织修复 |
Effect of nitric oxide synthase inhibitor on proteoglycan metabolism in repaired articular cartilage in rabbits |
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| SUN Wei ,JIN Da-di,WANG Ji-xing,QIN Li-yun and LIU Xiao-xia. Effect of nitric oxide synthase inhibitor on proteoglycan metabolism in repaired articular cartilage in rabbits[J]. Chinese journal of traumatology, 2003, 6(6): 336-340 | |
| Authors: | SUN Wei JIN Da-di WANG Ji-xing QIN Li-yun LIU Xiao-xia |
| Affiliation: | 1. Department of Traumatic Surgery, Shenzhen Second People's Hospital, Shenzhen 518025, China 2. Department of Orthopaedics & Spine Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China 3. Department of Surgery, 188th Hospital of PLA, Chaozhou 515000, China |
| Abstract: | OBJECTIVE: To study the effect of nitric oxide synthase inhibitor, S-methyl thiocarbamate (SMT), on proteoglycan metabolism in repaired articular cartilage in rabbits. METHODS: Twenty-four male New Zealand white rabbits, aged 8 months and weighing 2.5 kg+/-0.2 kg, were used in this study. Cartilage defects in full thickness were created on the intercondylar articular surface of bilateral femurs of all the rabbits. Then the rabbits were randomly divided into 3 groups (n=8 in each group). The defects in one group were filled with fibrin glue impregnated with recombinant human bone morphogenetic protein-2 (rhBMP-2, BMP group), in one group with fibrin glue impregnated with rhBMP-2 and hypodermic injection with SMT (SMT group) and in the other group with nothing (control group). All the animals were killed at one year postoperatively. The tissue sections were stained with safranine O-fast green and analyzed by Quantiment 500 system to determine the content of glycosaminoglycan through measuring the percentage of safranine O-stained area, the thickness of cartilages and the mean gray scale (average stain intensity). Radiolabelled sodium sulphate (Na(2)(35)SO(4)) was used to assess the proteoglycan synthesis. RESULTS: At one year postoperatively, the percentage of safranine O-stained area, the mean gray scale and the cartilage thickness of the repaired tissues in SMT group were significantly higher than those of BMP group (P<0.01) and the control group (P<0.05). Result of incorporation of Na(2)(35)SO(4) showed that the proteoglycan synthesis in SMT group was higher than those of BMP group and the control group (P<0.01). CONCLUSIONS: SMT, a nitric oxide synthase inhibitor, can significantly increase the content of glycosaminoglycan and proteoglycan synthesis, and computer-based image analysis is a reliable method for evaluating proteoglycan metabolism. |
| Keywords: | Nitric oxide Cartilage Glycosaminoglycans Proteoglycans S-methyl thiocarbamate |
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