川崎病患儿血清基质金属蛋白酶-1表达的意义

目的 探讨基质金属蛋白酶-1(MMP-1)在川崎病(KD)患儿冠状动脉损伤中的作用.方法 选择KD患儿36例(其中无冠状动脉损伤组15例,冠状动脉损伤组21例),按病程分为急性期、亚急性期和恢复期;10例健康儿童为健康对照组.应用酶联免疫吸附法检测其血清MMP-1蛋白水平.结果 KD急性期患儿血清MMP-1水平明显高于健康对照组(Pa＜0.01),且冠状动脉损伤组较无冠状动脉损伤组升高更显著(P＜0.05);亚急性期、恢复期其MMP-1水平明显降低(各期间比较Pa＜0.01).KD组急性期血清MMP-1水平与外周血白细胞计数呈显著正相关(r＝0.791 P＜0.01).结论 检测血清MMP-1水平对预测和早期诊断KD并冠状动脉病变具有重要意义.

Significance of Expression of Matrix Metalloproteinase 1 in Peripheral Blood of Children with Kawasaki Disease

Objective To explore the potential role of matrix metalloprotenase-1(MMP-1) in the pathogenesis of coronary artery lesions(CAL)in children with Kawasaki disease(KD).Methods Thirty-six children with KD,including 21 children without CAL and 15 children with CAL,as well as age-matched 10 healthy afebrile controls were studied.The courses of KD were divided into 3 phases:the acute phase,the subacute phase and the convalescent phase.Enzyme-linked immunosorbent assay was used to detect the protein level of MMP-1 in the sera.Results Levels of MMP-1 protein in serum were significantly elevated during the acute phase in 2 groups of KD children,compared with afebrile controls(Pa<0.01).Furthermore,MMP-1 protein level was significantly higher in KD patients with CAL than that in KD without CAL(P<0.05).There was significantly positive correlation between the serum level of MMP-1 in the acute phase of KD and the circulating leucocytes counts(r=0.791 P<0.01).MMP-1 serum protein level in the acute phase of the 2 KD groups decreased obviously from the subacute through the convalescent phases(P<0.01).Conclusion The measurement of serum MMP-1 may be of important clinical value in the prediction and early diagnosis of KD with coronary artery lesion.