谷氨酸对PC12细胞中磷酸化肌细胞增强因子2A蛋白表达的影响

目的研究兴奋性氨基酸谷氨酸对PC12细胞中磷酸化肌细胞增强因子2A(P-MEF2A)蛋白表达的影响与意义。方法体外培养PC12细胞,设立正常对照组,0.1、1.0、10.0 nmol/L谷氨酸处理组。Western blot测定P-MEF2A与突触素1的蛋白表达量,并进行2种蛋白表达相关性分析。结果谷氨酸诱导PC12细胞中P-MEF2A蛋白表达升高,呈剂量依赖性(F=51.54 P<0.01)。10.0 nmol/L谷氨酸处理24 h后,P-MEF2A蛋白的表达与突触素1的表达量呈显著负相关(r=-0.788 P<0.01)。结论谷氨酸诱导PC12细胞P-MEF2A表达升高,并与学习记忆调控相关基因突触素1的表达下调有关。

Effects of Glutamate on the Expression of Phosphorylated Myocyte Enhancer Factor 2A Protein in PC12 cells

Objective To investigate the effects of glutamate on the expression of phosphorylated myocyte enhancer factor 2A(P-MEF 2A) protein in PC12 cells.Methods PC12 cells were incubated and were divided into normal group,0.1 nmol/L,1.0 nmol/L,10.0 nmol/L glutamate treated group.Western blot was used to detect the protein levels of P-MEF2A and synapsin-1.Results Glutamate induced the increased of P-MEF2A protein levels in a dose-depended way(F=51.54 P<0.01).After PC12 cells were exposed to 10.0 nmol/L glutamate for 24 h,the expression levels of P-MEF2A protein were significantly inverse correlated with synapsin-1 protein levels(r =-0.788 P<0.01).Conclusions Glutamate induced the increase of P-MEF2A in PC12 cells.And it might be correlated with the decrease of synapsin-1 which is related with learning and memory.