补充VE、Se对大鼠肝纤维化和抗氧化功能影响的研究

目的 :　探讨维生素 E(VE)、硒 (Se)对 CCl4 大鼠肝纤维化和抗氧化功能的影响。方法 :　 48只 SD大鼠随机分为 3组。模型组 ,采用腹腔注射 5 0 % CCl4 和橄榄油混合制剂的方式建立大鼠肝纤维化模型 ,喂饲标准饲料 ;干预组 ,处理方式同前 ,并在大鼠饲料中加 VE(2 5 0 mg/kg )和 Se(0 .2 mg/kg)进行营养干预 ;对照组 ,腹腔注射生理盐水 ,喂饲标准饲料。全部动物于处理 8w后处死。通过 HE常规染色和 Masson三色胶原染色对肝组织切片进行病理组织学检查 ;同时分别检测大鼠体内各种抗氧化指标和其他肝纤维化指标 ,并观察其变化。结果 :　干预组大鼠抗氧化能力有显著提高 ,表现在肝组织和血清 SOD、红细胞 GPX活性均显著高于模型组 ,而肝组织和血清MDA水平显著低于模型组。同时 ,干预组大鼠的肝纤维化程度显著低于模型组 ,体现在血清 ALT、AST水平、肝组织羟脯氨酸含量都显著低于模型组 ;组织病理学检查显示肝脏胶原纤维增生程度也显著低于模型组。结论 :　补充适量 VE和 Se具有提高机体抗氧化的能力 ,显著减轻肝脏胶原纤维的增生程度

EFFECT OF VITAMIN E AND SELENIUM ON LIVER FIBROSIS AND ANTIOXIDATIVE FUNCTION IN CARBON TETRACHLORIDE-INDUCED RATS

Objective: To investigate the effect of vitamin E (VE) and selenium (Se) on liver fibrosis and antioxidative function in the carbon tetrachloride induced rats. Methods: 48 normal male SD rats were randomly divided into three groups (16 rats / group): intervention group, pathological group and control group. The control group was injected with normal saline; the others were given intraperitonally CCl 4 (diluted with an equal volume of olive oil). The rats in the intervention group were fed with chow supplemented with VE (250 mg/kg) and Se(0.2 mg/kg), and the others were given standard chow. All rats were put to death after 8 w injection. Tissue sections were stained with routine HE and Masson trichrome collagen; the markers of liver fibrosis and antioxidative function were detected and the changes of these markers were observed. Results: As compared with rats in pathological group, a lower degree of fiber proliferation occurred in the rats in intervention group. The serum levels of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) and the content of hydroxyproline in the liver tissue were significantly lower; the rats in intervention group had a higher ability of anti oxidation, and the activity of SOD (superoxide dismutase) in the liver tissue and serum and GPX (glutathione peroxidase) in erythrocyte were higher, and the MDA (malondialdehyde) levels in tissue and serum levels were significantly lower. Conclusion: The adequate dietary supplement of VE and selenium could elevate the ability of anti oxidation and the proliferative degree of collagenous fibers in liver was significantly reduced.