Expression of mTOR signaling pathway markers in prostate cancer progression |
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Authors: | Kremer Celeste L Klein Rob R Mendelson Jenny Browne Walden Samadzedeh Linda K Vanpatten Kristie Highstrom Lindsey Pestano Gary A Nagle Raymond B |
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Affiliation: | Arizona Cancer Center, University of Arizona, 1525 N. Campbell Avenue, Tucson, AZ 85724, USA. |
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Abstract: | BACKGROUND: The PI3K/AKT/mTOR pathway is central to prostate cancer progression. A preliminary investigation of immuno-histochemical expression of mammalian target of rapamycin (mTOR) pathway markers was undertaken to identify patterns of expression in prostate tissue. METHODS: Immunohistochemistry was performed on a custom-made prostate tissue array. Mean long scores and variability of long scores for each marker were recorded for normal lumenal cells, prostate intraepithelial neoplasia (PIN), and cancer. RESULTS: Expression of PTEN decreased and mTOR signaling pathway markers increased in PIN and in cancer as compared to normal cells in the majority of samples. Overexpression of 4E-BP1 and p-4E-BP1 was observed in PIN and cancer. However, in cancer, the overexpression of 4E-BP1 was significantly higher than with any other marker. DISCUSSION: Results suggest that 4E-BP1 overexpression is strongly associated with prostate cancer, especially when combined with PTEN and mTOR expression data. Hierarchical clustering analysis utilizing PTEN, mTOR, and 4E-BP1 separated normal from cancer cell populations in most cases. |
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Keywords: | prostate tissue array mTOR PTEN 4E‐BP1 prostate cancer |
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